公开(公告)号：US20100168390A1

公开(公告)日：2010-07-01

申请号：US12644554

申请日：2009-12-22

申请人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jorgen Scholler ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

发明人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jorgen Scholler ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

IPC分类号： C07K14/74

CPC分类号： C07K14/70539 ,  A61K38/00 ,  A61K47/61 ,  A61K47/6425 ,  A61K47/665 ,  B82Y5/00 ,  Y02A50/55 ,  Y02A50/57 ,  Y02A50/58

摘要： The present invention describes novel methods to generate MHC multimers and methods to improve existing and new MHC multimers. The invention also describes improved methods for the use of MHC multimers in analysis of T-cells in samples including diagnostic and prognostic methods. Furthermore the use of MHC multimers in therapy are described, e.g. anti-tumour and anti-virus therapy, including isolation of antigen specific T-cells capable of inactivation or elimination of undesirable targeT-cells or isolation of specific T-cells capable of regulation of other immune cells.

摘要翻译： 本发明描述了生成MHC多聚体的新方法和改进现有的和新的MHC多聚体的方法。 本发明还描述了在样品中T细胞分析中使用MHC多聚体的改进方法，包括诊断和预后方法。 此外，MHC多聚体在治疗中的使用描述如下。 抗肿瘤和抗病毒治疗，包括分离能够灭活或消除不需要的targeT细胞的抗原特异性T细胞或分离能够调节其他免疫细胞的特异性T细胞。

公开(公告)号：US10030065B2

公开(公告)日：2018-07-24

申请号：US12644554

申请日：2009-12-22

申请人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jørgen Schøller ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

发明人： Liselotte Brix ,  Henrik Pedersen ,  Tina Jakobsen ,  Jørgen Schøller ,  Jesper Lohse ,  Katja Brunstedt ,  Kivin Jacobsen

IPC分类号： C07K14/74 ,  A61K47/61 ,  A61K47/64 ,  A61K47/66 ,  B82Y5/00 ,  A61K38/00

摘要： The present invention relates to a soluble negative control MHC multimer comprising a nonsense peptide that binds the MHC protein efficiently, but that does not support binding of the resultant MHC-peptide complex to the desired T Cell Receptor. The nonsense peptide is designed to i) have a length enabling binding to the MHC allele in question, ii) have appropriate amino acids at relevant anchor positions which anchor the nonsense peptide to the peptide-binding groove of the MHC, iii) have amino acids outside the anchor positions that do not support binding to a T Cell Receptor, and iv) have an amino acid sequence that is different from the linear sequence of any naturally occurring peptide.