公开(公告)号：US5418129A

公开(公告)日：1995-05-23

申请号：US803065

申请日：1991-12-06

申请人： Edward Nudelman ,  Anil Singhal ,  Henrik Clausen ,  Sen-itiroh Hakomori ,  Kazuo Muroi ,  Toshio Suda ,  Hisao Nojiri

发明人： Edward Nudelman ,  Anil Singhal ,  Henrik Clausen ,  Sen-itiroh Hakomori ,  Kazuo Muroi ,  Toshio Suda ,  Hisao Nojiri

IPC分类号： A61K38/00 ,  C07K16/30 ,  C12P21/08 ,  A01N1/02 ,  A61K39/395 ,  C07K15/28

CPC分类号： C07K16/30 ,  A61K38/00

摘要： A method of producing monoclonal antibodies that bind to tumor-associated gangliosides, the method comprising: (1) immunizing a host with tumor cells; (2) boosting the host with a suspension comprising a mixture of tumor cell membrane and at least one purified lactonized tumor-associated ganglioside; (3) boosting the host with an immunogen comprising a lactone of a tumor associated ganglioside adsorbed on or incorporated into a carrier; (4) fusing immunized cells from the host with myeloma cells to form hybridoma cells; (5) selecting hybridoma cells that produce antibody that binds to the ganglioside of step (3); (6) culturing the selected hybridoma cells; and (7) recovering the antibody.

摘要翻译： 一种产生与肿瘤相关神经节苷脂结合的单克隆抗体的方法，所述方法包括：（1）用肿瘤细胞免疫宿主; （2）用包含肿瘤细胞膜和至少一种经内化的内源性肿瘤相关神经节苷脂的混合物的悬浮液加强宿主; （3）用包含吸附在载体上或结合到载体中的肿瘤相关神经节苷脂的内酯的免疫原来加强宿主; （4）将来自宿主的免疫细胞与骨髓瘤细胞融合以形成杂交瘤细胞; （5）选择产生与步骤（3）的神经节苷脂结合的抗体的杂交瘤细胞; （6）培养所选择的杂交瘤细胞; 和（7）回收抗体。