公开(公告)号：US06495675B1

公开(公告)日：2002-12-17

申请号：US09603378

申请日：2000-06-26

申请人： Hiroshi Takaku ,  Kin-ichiro Miura ,  Toshifumi Hatta ,  Kazuyuki Takai ,  Masahide Ishikawa

发明人： Hiroshi Takaku ,  Kin-ichiro Miura ,  Toshifumi Hatta ,  Kazuyuki Takai ,  Masahide Ishikawa

IPC分类号： C07H2104

CPC分类号： A61K31/7105 ,  A61K38/00 ,  C07H21/00 ,  C12N15/1131 ,  C12N2310/317 ,  C12N2310/321 ,  C12N2310/3521

摘要： A pharmaceutical composition comprising a capped oligonucleotide capable of binding to a PB2 protein of an influenza virus RNA polymerase, and a pharmaceutically acceptable carrier or dilute, said capped oligonucleotide having a structure of the formula (I): m7GpppXpY  (I) wherein ppp is a triphosphate bridge, p is a monophosphate bridge, m7G is 7-methylguanosine group binding at 5′ end thereof to said ppp, X is a 2′-O-methyl guanosine group or guanosine group, said 2′-O-methyl guanosine group or guanosine group binds at a 5′ end thereof to said ppp, and at a 3′ end thereof to said p, and Y is an oligoribonucleotide moiety binding at 5′ end thereof to said p, and having 5 to 11 bases is disclosed. The pharmaceutical composition can inhibit a protein expression of an influenza virus.

摘要翻译： 一种药物组合物，其包含能够结合流感病毒RNA聚合酶的PB2蛋白的封端寡核苷酸和药学上可接受的载体或稀释的具有式（I）结构的所述封端寡核苷酸：其中ppp是三磷酸桥，p 是单磷酸桥，m7G是在5'末端与所述ppp结合的7-甲基鸟苷类，X是2'-O-甲基鸟苷或鸟苷酸，所述2'-O-甲基鸟苷或鸟苷基结合于 其5'端至所述ppp，并且在其3'末端与所述p结合，Y是在其5'末端与所述p结合并且具有5至11个碱基的寡核糖核苷酸部分。 药物组合物可以抑制流感病毒的蛋白质表达。