公开(公告)号：US20110105734A1

公开(公告)日：2011-05-05

申请号：US12517936

申请日：2006-12-19

申请人： Atsuko Kawasaki ,  Keisuke Mukai ,  Sei Kirihara

发明人： Atsuko Kawasaki ,  Keisuke Mukai ,  Sei Kirihara

IPC分类号： C12P21/00 ,  C07K1/22

CPC分类号： C07K14/505

摘要： Disclosed is a method for production of human erythropoietin. By the method, the cells are cultured in a serum-free medium with repetitive medium exchanges, in which medium exchange is carried out either by collecting 80 to 95% of the culture when viable cell density has reached at 2×106˜4×106 cells/mL, or by adjusting the amount of the exchanged medium so that the initial density of the viable cells may be 1.5×105˜2.5×105 cells/mL.

摘要翻译： 公开了一种生产人促红细胞生成素的方法。 通过该方法，将细胞在具有重复培养基交换的无血清培养基中培养，当存活细胞密度达到2×106〜4×106时，通过收集80〜95％的培养物进行培养基交换 细胞/ mL，或通过调节交换培养基的量使活细胞的初始密度为1.5×105〜2.5×10 5个细胞/ mL。