公开(公告)号：US5998178A

公开(公告)日：1999-12-07

申请号：US452075

申请日：1995-05-26

申请人： Ken-ichi Hashiguchi ,  Hiroko Kishino ,  Nobuharu Tsujimoto ,  Hiroshi Matsui

发明人： Ken-ichi Hashiguchi ,  Hiroko Kishino ,  Nobuharu Tsujimoto ,  Hiroshi Matsui

IPC分类号： C12N1/21 ,  C12N15/52 ,  C12P13/06

CPC分类号： C12P13/06 ,  C12N15/52

摘要： An L-isoleucine-producing bacterium belonging to the genus Escherichia which carries a thrABC operon which comprises a thrA gene coding for aspartokinase I-homoserine dehydrogenase I substantially released from the inhibition by L-threonine and an ilvGMEDA operon which comprises an ilvA gene coding for threonine deaminase substantially released from the inhibition by L-isoleucine and whose region required for attenuation is removed; and an L-isoleucine-producing bacterium belonging to the genus Escherichia carrying the thrABC operon, an lysC gene coding for aspartokinase III substantially released from the inhibition by L-lysine, and the ilvGMEDA operon. The bacteria permit biosynthesis of a sufficient amount of L-isoleucine.

摘要翻译： 属于埃希氏杆菌属的属于埃希氏杆菌属的L-异亮氨酸细菌，其携带thrABC操纵子，其包含基本上由L-苏氨酸抑制的编码天冬氨酸激酶I-高丝氨酸脱氢酶I的thrA基因，以及ilvGMEDA操纵子，该ilvGMEDA操纵子包含编码 苏氨酸脱氨酶基本上从L-异亮氨酸的抑制中释放出去除其衰减所需的区域; 以及属于携带thrABC操纵子的埃希氏菌属的L-异亮氨酸生产细菌，基本上由L-赖氨酸的抑制释放的天冬氨酸激酶III的lysC基因和ilvGMEDA操纵子。 细菌允许生物合成足量的L-异亮氨酸。