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1.发明授权L-isoleucine-producing bacterium and method for preparing L-isoleucine through fermentation 失效L-异亮氨酸生产菌和通过发酵制备L-异亮氨酸的方法
公开(公告)号：US5998178A
公开(公告)日：1999-12-07
申请号：US452075
申请日：1995-05-26
摘要： An L-isoleucine-producing bacterium belonging to the genus Escherichia which carries a thrABC operon which comprises a thrA gene coding for aspartokinase I-homoserine dehydrogenase I substantially released from the inhibition by L-threonine and an ilvGMEDA operon which comprises an ilvA gene coding for threonine deaminase substantially released from the inhibition by L-isoleucine and whose region required for attenuation is removed; and an L-isoleucine-producing bacterium belonging to the genus Escherichia carrying the thrABC operon, an lysC gene coding for aspartokinase III substantially released from the inhibition by L-lysine, and the ilvGMEDA operon. The bacteria permit biosynthesis of a sufficient amount of L-isoleucine.
摘要翻译： 属于埃希氏杆菌属的属于埃希氏杆菌属的L-异亮氨酸细菌，其携带thrABC操纵子，其包含基本上由L-苏氨酸抑制的编码天冬氨酸激酶I-高丝氨酸脱氢酶I的thrA基因，以及ilvGMEDA操纵子，该ilvGMEDA操纵子包含编码 苏氨酸脱氨酶基本上从L-异亮氨酸的抑制中释放出去除其衰减所需的区域; 以及属于携带thrABC操纵子的埃希氏菌属的L-异亮氨酸生产细菌，基本上由L-赖氨酸的抑制释放的天冬氨酸激酶III的lysC基因和ilvGMEDA操纵子。 细菌允许生物合成足量的L-异亮氨酸。
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公开(公告)号：US5573945A
公开(公告)日：1996-11-12
申请号：US370193
申请日：1995-01-09
申请人： Eiji Ono , Nobuharu Tsujimoto , Kazuhiko Matsui , Osamu Kurahashi
发明人： Eiji Ono , Nobuharu Tsujimoto , Kazuhiko Matsui , Osamu Kurahashi
摘要： A mutant of the genus Escherichia is described, the .alpha.-ketoglutarate dehydrogenase activity of which is deficient or reduced, and/or the phosphoenol pyruvate carboxylase and/or glutamate dehydrogenase activities of which are amplified. The mutant is useful in the fermentative production of L-glutamic acid.
摘要翻译： 描述了埃希氏菌属的突变体，α-酮戊二酸脱氢酶活性缺乏或减少，和/或磷酸烯醇丙酮酸羧化酶和/或谷氨酸脱氢酶活性被扩增。 该突变体可用于L-谷氨酸的发酵生产。
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3.发明授权公开(公告)号：US07211421B2
公开(公告)日：2007-05-01
申请号：US11073741
申请日：2005-03-08
摘要： An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase (named dapB) is obtained from the transformant.
摘要翻译： 用甲醇梭菌的染色体基因文库转化二氢吡啶二羧酸合酶或二氢吡啶二羧酸还原酶缺陷的大肠杆菌突变菌株，选择能在最小培养基上生长的转化株。 从转化体获得编码二氢吡啶二酸合酶或二氢吡啶二羧酸还原酶（命名为dapB）的重组DNA。
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4.发明申请公开(公告)号：US20080038825A1
公开(公告)日：2008-02-14
申请号：US11738617
申请日：2007-04-23
申请人： Yoshiya Gunji , Hisashi Yasueda , Shinichi Sugimoto , Nobuharu Tsujimoto , Megumi Shimaoka , Yuri Miyata , Manami Oba
发明人： Yoshiya Gunji , Hisashi Yasueda , Shinichi Sugimoto , Nobuharu Tsujimoto , Megumi Shimaoka , Yuri Miyata , Manami Oba
CPC分类号： C12P13/08 , C12N9/1217 , C12N9/88 , C12P13/04 , C12R1/01
摘要： An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as the main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation of cells with a DNA coding for dihydrodipicolinate synthase that is desensitized to feedback inhibition by L-lysine and a DNA coding for aspartokinase that is desensitized to feedback inhibition by L-lysine, or a Methylophilus bacterium which is casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.
摘要翻译： 通过培养可以通过使用甲醇作为主要碳源生长并且具有L-氨基酸生产能力的甲型幽门螺杆菌细菌产生L-氨基酸，例如其中二氢吡啶二酸合成酶活性和天冬氨酸激酶活性被增强的甲基幽门螺杆菌 将编码二脱氧叶酸二磷酸合酶的DNA转化为L-赖氨酸的反馈抑制的脱氧赖氨酸的编码天冬氨酸激酶的DNA或L-赖氨酸反馈抑制的天冬氨酸的DNA或作为酪蛋白氨基酸营养缺陷型的细鳞状芽孢杆菌属细菌的DNA在培养基 含有甲醇作为主要碳源，在培养中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。
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5.发明授权Methylophilus methylotrophus having enhanced dihydrodipicolinate synthase and/or aspartokinase activity for L-amino acid production 有权具有增强的二氢吡啶二酸合成酶和/或L-氨基酸生产的天冬氨酸激酶活性的甲基嗜甲基病毒公开(公告)号：US07223572B1
公开(公告)日：2007-05-29
申请号：US09926299
申请日：2000-04-07
申请人： Yoshiya Gunji , Hisashi Yasueda , Shinichi Sugimoto , Nobuharu Tsujimoto , Megumi Shimaoka , Yuri Miyata , Manami Oba
发明人： Yoshiya Gunji , Hisashi Yasueda , Shinichi Sugimoto , Nobuharu Tsujimoto , Megumi Shimaoka , Yuri Miyata , Manami Oba
CPC分类号： C12P13/08 , C12N9/1217 , C12N9/88 , C12P13/04 , C12R1/01
摘要： An L-amino acid is produced by culturing a Methylophilus bacterium which can grow by using methanol as a main carbon source and has L-amino acid-producing ability, for example, a Methylophilus bacterium in which dihydrodipicolinate synthase activity and aspartokinase activity are enhanced by transformation through introduction into cells, of a DNA coding for dihydrodipicolinate synthase that does not suffer feedback inhibition by L-lysine and a DNA coding for aspartokinase that does not suffer feedback inhibition by L-lysine, or a Methylophilus bacterium made to be casamino acid auxotrophic, in a medium containing methanol as a main carbon source, to produce and accumulate an L-amino acid in culture, and collecting the L-amino acid from the culture.
摘要翻译： 通过培养可以通过使用甲醇作为主要碳源生长并具有L-氨基酸生产能力的甲型幽门螺杆菌细菌产生L-氨基酸，例如其中二氢吡啶二羧酸合酶活性和天冬氨酸激酶活性被增强的甲基幽门螺杆菌 通过引入细胞的转化，编码不受L-赖氨酸的反馈抑制的二氢吡啶并吡啶合成酶的DNA和编码不受L-赖氨酸的反馈抑制的天冬氨酸激酶的DNA，或被制备为酪氨酸氨基酸营养缺陷型的甲型幽门螺杆菌 在含有甲醇作为主要碳源的介质中，在培养物中产生和积累L-氨基酸，并从培养物中收集L-氨基酸。
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6.发明授权Method for producing target substance using microorganisms with reduced interactions between MalK and IIAGlc 有权使用具有降低的MalK和IIAGlc相互作用的微生物生产目标物质的方法公开(公告)号：US07097999B2
公开(公告)日：2006-08-29
申请号：US10121693
申请日：2002-04-15
申请人： Nobuharu Tsujimoto , Tomoko Suzuki , Hisao Ito
发明人： Nobuharu Tsujimoto , Tomoko Suzuki , Hisao Ito
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08 , C12P13/222
摘要： The present invention describes a method for producing a target substance by utilizing a microorganism comprising culturing the microorganism in a medium, allowing the target substance to accumulate, and collecting the target substance from the medium. Also the microorganism used in the present invention is a mutant strain whereby maltose assimilation is controlled by the interaction between IIAGlc protein of glucose PTS and MalK.
摘要翻译： 本发明描述了通过利用微生物来生产目标物质的方法，所述微生物包括在培养基中培养微生物，允许目标物质积聚，并从培养基中收集目标物质。 本发明中使用的微生物也是通过葡萄糖PTS和MalK的IIA蛋白质蛋白质之间的相互作用来控制麦芽糖同化的突变菌株。
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公开(公告)号：US5977331A
公开(公告)日：1999-11-02
申请号：US750152
申请日：1996-12-12
申请人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Yoshihiro Usuda , Nobuharu Tsujimoto , Eiichiro Kimura , Chizu Abe , Yoshio Kawahara , Tsuyoshi Nakamatsu , Osamu Kurahashi
CPC分类号： C12N9/0008 , C12P13/08 , C12P13/14
摘要： Disclosed are coryneform L-glutamic acid-producing bacteria deficient in .alpha.-ketoglutarate dehydrogenase, a method of producing L-glutamic acid by using the bacteria, a gene coding for an enzyme having .alpha.-KGDH activity originating from coryneform L-glutamic acid-producing bacteria, recombinant DNA containing the gene, coryneform bacteria harboring the recombinant DNA, and a method of producing L-lysine by using bacteria harboring the recombinant DNA and having L-lysine productivity.
摘要翻译： PCT No.PCT / JP95 / 01131 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月7日PCT公布。 WO95 / 34672 PCT公开号 日期1995年12月21日公开是α-酮戊二酸脱氢酶缺乏的棒状L-谷氨酸生产菌，使用细菌生产L-谷氨酸的方法，编码具有源自棒状杆菌L的α-KGDH活性的酶的基因 谷氨酸生产菌，含有该基因的重组DNA，携带重组DNA的棒状细菌，以及通过使用含有重组DNA并具有L-赖氨酸生产力的细菌产生L-赖氨酸的方法。
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公开(公告)号：US08497087B2
公开(公告)日：2013-07-30
申请号：US11275900
申请日：2006-02-02
申请人： Nobuharu Tsujimoto , Tomoko Suzuki , Hisao Ito
发明人： Nobuharu Tsujimoto , Tomoko Suzuki , Hisao Ito
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08 , C12P13/222
摘要： A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is controlled by reducing or eliminating the interaction between IIAGlc protein of the glucose PTS and a protein involved in non-PTS uptake of maltose.
摘要翻译： 描述了通过在培养基中培养微生物来生产和积累目标物质并从培养物中收集目标物质的微生物生产目标物质的方法。 该微生物是通过减少或消除葡萄糖PTS的IIAGlc蛋白与参与非PTS摄取麦芽糖的蛋白质之间的相互作用来控制麦芽糖同化的突变重组菌株。
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9.发明授权公开(公告)号：US06878533B2
公开(公告)日：2005-04-12
申请号：US10214556
申请日：2002-08-09
IPC分类号： C12N15/09 , C07H21/04 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N9/06 , C12N9/88 , C12N15/53 , C12N15/60 , C12P13/08 , C12R1/07
摘要： An Escherichia coil mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library of Bacillus methanolicus, a transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.
摘要翻译： 通过使用甲醇梭菌的染色体基因文库转化缺乏二氢吡啶二羧酸合酶或二氢吡啶二羧酸还原酶的大肠杆菌突变株，选择可在基本培养基上生长的转化体菌株，以及含有编码二氢吡啶二羧酸合酶或二氢吡啶二羧酸还原酶的DNA的重组DNA 从转化体获得。
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公开(公告)号：US20060205043A1
公开(公告)日：2006-09-14
申请号：US11275900
申请日：2006-02-02
申请人： Nobuharu Tsujimoto , Tomoko Suzuki , Hisao Ito
发明人： Nobuharu Tsujimoto , Tomoko Suzuki , Hisao Ito
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08 , C12P13/222
摘要： A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is controlled by reducing or eliminating the interaction between IIAGlc protein of the glucose PTS and a protein involved in non-PTS uptake of maltose.
摘要翻译： 描述了通过在培养基中培养微生物来生产和积累目标物质并从培养物中收集目标物质的微生物生产目标物质的方法。 微生物是通过减少或消除葡萄糖PTS的IIA蛋白与参与非PTS摄取麦芽糖的蛋白质之间的相互作用来控制麦芽糖同化的突变重组菌株。
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