Primers and method of detecting bacteria
    1.
    发明授权
    Primers and method of detecting bacteria 有权
    引物和检测细菌的方法

    公开(公告)号:US07326779B2

    公开(公告)日:2008-02-05

    申请号:US10491449

    申请日:2002-10-11

    IPC分类号: C07H21/04 C12Q1/68 C12P19/34

    摘要: The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

    摘要翻译: 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌,沙门氏菌和弧菌属细菌的16S rRNA编码DNA,但在相同条件下用于PCR时, 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA,但是当在相同条件下用于PCR时,不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

    Primers and method of detecting bacteria
    2.
    发明申请
    Primers and method of detecting bacteria 有权
    引物和检测细菌的方法

    公开(公告)号:US20050176001A1

    公开(公告)日:2005-08-11

    申请号:US10491449

    申请日:2002-10-11

    摘要: The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

    摘要翻译: 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌,沙门氏菌和弧菌属细菌的16S rRNA编码DNA,但在相同条件下用于PCR时, 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA,但是当在相同条件下用于PCR时,不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。