公开(公告)号：US08329406B2

公开(公告)日：2012-12-11

申请号：US12524735

申请日：2008-01-30

申请人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

发明人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C07K14/415

摘要： Provided is a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut. The method involves a PCR method for specifically detecting nuts of the family Juglandaceae and identifying the matK sequence of pecan nut to thereby discriminate walnut and pecan nut from each other optionally using restriction enzymes.

摘要翻译： 提供了一种核桃可以简单，快速和精确地分离和区别于山核桃坚果的方法。 该方法涉及用于特异性检测菊科植物的坚果的PCR方法，并鉴定山核桃坚果的matK序列，从而可选地使用限制性内切酶核桃和山核桃果仁。

公开(公告)号：US07326779B2

公开(公告)日：2008-02-05

申请号：US10491449

申请日：2002-10-11

申请人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

发明人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

摘要翻译： 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌，沙门氏菌和弧菌属细菌的16S rRNA编码DNA，但在相同条件下用于PCR时， 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA，但是当在相同条件下用于PCR时，不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

公开(公告)号：US20050176001A1

公开(公告)日：2005-08-11

申请号：US10491449

申请日：2002-10-11

申请人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

发明人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

IPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

摘要翻译： 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌，沙门氏菌和弧菌属细菌的16S rRNA编码DNA，但在相同条件下用于PCR时， 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA，但是当在相同条件下用于PCR时，不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

公开(公告)号：US06391439B2

公开(公告)日：2002-05-21

申请号：US08904301

申请日：1997-07-31

申请人： Shigeru Nakano

发明人： Shigeru Nakano

IPC分类号： B41M500

CPC分类号： G09F3/02 ,  G09F3/10 ,  G11B5/72 ,  G11B23/40 ,  Y10T428/249997 ,  Y10T428/2848

摘要： A rewritable indication device layer on which visible information is rewritably recorded and erased, is formed on a label base layer. A protection layer for protecting the rewritable indication device layer is formed on the rewritable indication device layer. The rewritable indication label for a recording medium having such structure, is adhered to a recording medium or a recording medium case.

公开(公告)号：US6127579A

公开(公告)日：2000-10-03

申请号：US345980

申请日：1999-07-01

申请人： Shigeru Nakano ,  Noriko Yoneta ,  Takashi Tate

发明人： Shigeru Nakano ,  Noriko Yoneta ,  Takashi Tate

IPC分类号： C07C41/54 ,  C07C41/24 ,  C07C43/303 ,  C07C43/305 ,  C07C45/42 ,  C07C45/51 ,  C07C45/60 ,  C07C49/67 ,  C07D309/12 ,  C07C45/30 ,  C07C43/02

CPC分类号： C07D309/12 ,  C07C41/03 ,  C07C41/18 ,  C07C41/48 ,  C07C41/54 ,  C07C45/513 ,  C07C45/515 ,  C07C45/60 ,  C07C2102/08

摘要： A method of manufacturing the useful substance 1-indanone from easily obtainable materials consists of reacting an indanyl ether represented by the generic formula ##STR1## under basic conditions to give a novel substance which is the indenyl ether derivative represented by the generic formula ##STR2## and hydrolyzing this to give the 1-indanone represented by the generic formula ##STR3##

摘要翻译： 从容易获得的材料制备有用物质1-二氢茚酮的方法包括在碱性条件下使由通式表示的茚满基醚与通式表示的茚基醚衍生物的新物质反应并水解，得到 由通式表示的1-二氢茚酮

公开(公告)号：US5024284A

公开(公告)日：1991-06-18

申请号：US384206

申请日：1989-05-31

申请人： Shigeru Nakano ,  Yasuhiro Kondou

发明人： Shigeru Nakano ,  Yasuhiro Kondou

IPC分类号： B60K31/04 ,  B60W30/00 ,  F02D29/02

CPC分类号： B60K31/047

摘要： The present invention relates to an apparatus for running a car at constant speed, the apparatus controlling the car to run at a desired speed, wherein by a control circuit (21), both a car-speed deviation and an acceleration are taken as variables, and a pulse-width of pulse signals having a predetermined period is computed from a function for defining pulse-width, and the above-mentioned pulse is given to an actuator (12) for adjusting opening of a throttle valve (13), thereby quickening to settle down to a set car-speed, to enable to stably run at constant speed. Moreover, resetting device (9), (220), second constant-speed-running canceling device (11), (219) which cancel a state of constant-speed-running only by operation of a canceling switch (8), (28), and checking device (10) for checking memory data are provided, thereby improving safety and reliability of control.

摘要翻译： 本发明涉及一种以恒定速度运行轿厢的装置，该装置控制轿厢以期望的速度运行，其中，通过控制电路（21），将车速偏差和加速度都视为变量， 并且根据用于确定脉冲宽度的功能计算出具有预定周期的脉冲信号的脉冲宽度，并且将上述脉冲提供给用于调节节流阀（13）的开度的致动器（12），从而加速 以稳定的速度下车，以稳定的速度稳定运行。 另外，仅通过消除开关（8）的动作来取消恒速运行状态的复位装置（9），（220），第二恒速运行取消装置（11），（219），（28） ）和用于检查存储器数据的检查装置（10），从而提高控制的安全性和可靠性。

公开(公告)号：US4741960A

公开(公告)日：1988-05-03

申请号：US10957

申请日：1987-02-02

申请人： Hitoshi Takita ,  Nobuo Ishikawa ,  Shigeru Nakano

发明人： Hitoshi Takita ,  Nobuo Ishikawa ,  Shigeru Nakano

IPC分类号： G11B5/708 ,  C09D101/18 ,  C09D175/04 ,  C10M105/76 ,  C10N40/18 ,  G11B5/71 ,  G11B5/735 ,  G11B5/70

CPC分类号： G11B5/735 ,  Y10S428/90 ,  Y10T428/265 ,  Y10T428/31609 ,  Y10T428/31663 ,  Y10T428/31786

摘要： A magnetic recording medium comprising a back coat layer formed on a non-magnetic support on one side which is opposite to a side where a magnetic recording layer is formed. The back coat layer is made of a dispersion of a fluorine-modified organosiloxane in a binder resin. The organosiloxane is of the following formula ##STR1## in which R.sub.1 represents a saturated or unsaturated monohydrocarbon group having from 7 to 21 carbon atoms, R.sub.2 represents a saturated monohydrocarbon group having from 1 to 4 carbon atoms, k is an integer of from 1 to 12, l is an integer of from 2 to 6, m is an integer of 1 or 2, n is an integer of 1 or 2 provided that m+n.ltoreq.3.

摘要翻译： 一种磁记录介质，包括在与形成磁记录层的一侧相对的一侧上的非磁性载体上形成的背涂层。 背涂层由粘合剂树脂中的氟改性有机硅氧烷的分散体构成。 有机硅氧烷具有下式（A），其中R 1表示具有7至21个碳原子的饱和或不饱和单烃基，R 2表示具有1至4个碳原子的饱和单烃基，k是 1至12，l是2至6的整数，m是1或2的整数，n是1或2的整数，条件是m + n <3。

公开(公告)号：US07446192B2

公开(公告)日：2008-11-04

申请号：US10507446

申请日：2003-03-12

申请人： Hidetsugu Goto ,  Shigeru Nakano

发明人： Hidetsugu Goto ,  Shigeru Nakano

IPC分类号： C07H21/04

CPC分类号： C12J1/04 ,  C07K14/195 ,  C12N1/20

摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.

摘要翻译： 本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法 通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。 在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因 乙酸浓度。 此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度 也可以改善，而且可以显着提高最终的乙酸浓度。

公开(公告)号：US20080138904A1

公开(公告)日：2008-06-12

申请号：US11793145

申请日：2005-12-12

申请人： Shigeru Nakano ,  Masahiro Fukaya

发明人： Shigeru Nakano ,  Masahiro Fukaya

IPC分类号： C12N15/09 ,  C12N1/21

CPC分类号： C12P7/40 ,  C12N1/00 ,  C12N15/52 ,  C12P7/52 ,  C12P7/54

摘要： It is intended to provide a method of conferring to microbial cells or the like, tolerance to a short chain fatty acid, including formic acid and acetic acid, which is harmful to the growth thereof without suppressing the growth of the cells while maintaining a high productivity of useful substances. It is also intended to provide a means for efficiently culturing a microorganism in the presence of a short chain fatty acid, or a means for producing a useful short chain fatty acid via fermentation.The present invention provides a method of breeding cells to improve tolerance to a short chain fatty acid, wherein a gene encoding a protein having a function of transporting a short chain fatty acid from the inside of cells to the outside of cells, is transformed into and expressed in the cells; a high cell-density culture method for producing useful substances using the cell; and a method of preparing a fermentation broth comprising a short chain fatty acid, wherein the cells are cultured under the conditions such that the cells produce a short chain fatty acid.

摘要翻译： 旨在提供赋予微生物细胞等的方法，对包含甲酸和乙酸的短链脂肪酸的耐受性，其对生长有害，而不抑制细胞生长，同时保持高生产率 的有用物质。 还提供了在短链脂肪酸存在下有效培养微生物的方法，或者是通过发酵生产有用的短链脂肪酸的方法。 本发明提供一种育种细胞以改善对短链脂肪酸耐受性的方法，其中将编码具有从细胞内部输送到外部的短链脂肪酸的功能的蛋白质的基因转化到细胞外， 在细胞中表达; 用于使用该细胞产生有用物质的高细胞密度培养方法; 以及制备包含短链脂肪酸的发酵液的方法，其中所述细胞在使细胞产生短链脂肪酸的条件下培养。

公开(公告)号：US20070088088A1

公开(公告)日：2007-04-19

申请号：US11406339

申请日：2006-04-19

申请人： Yoichi Inada ,  Shigeru Nakano ,  Hiroaki Masuzaki

发明人： Yoichi Inada ,  Shigeru Nakano ,  Hiroaki Masuzaki

IPC分类号： A61K31/192

CPC分类号： A61K31/192

摘要： A method for preventing or treating metabolic syndrome by administering bezafibrate. Since bezafibrate suppresses the action of 11β-hydroxysteroid dehydrogenase type 1 and also accelerates expression of adiponectin receptor, it is used as an agent for preventing or treating metabolic syndrome.

摘要翻译： 一种通过施用苯扎贝特来预防或治疗代谢综合征的方法。 由于苯扎贝特抑制了1型11β-羟类固醇脱氢酶的作用，并且加速了脂连蛋白受体的表达，因此用作预防或治疗代谢综合征的药剂。