公开(公告)号：US5019498A

公开(公告)日：1991-05-28

申请号：US162672

申请日：1988-03-01

申请人： Kenji Chichibu

发明人： Kenji Chichibu

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N33/54393 ,  G01N33/5308 ,  G01N33/6887 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/815

摘要： A high molecular hyaluronic acid which is an important factor in the diagnosis of inflammations such as rheumatism or diseases such as cancer is assayed as a complex of sandwich structure in which a hyaluronic acid binding protein is coupled to the hyaluronic acid of interest at two or more sites of binding without the need to employ a competitive reaction as in the prior art techniques of assay. The assay method of the present invention does not require a purified form of hyaluronic acid as a reagent and permits as small as 10 ng of a high molecular hyaluronic acid to be detected or quantified by a very simple operation.

公开(公告)号：US4091089A

公开(公告)日：1978-05-23

申请号：US672497

申请日：1976-03-31

申请人： Kenji Chichibu ,  Kiyoshige Wakabayashi

发明人： Kenji Chichibu ,  Kiyoshige Wakabayashi

IPC分类号： G01N33/536 ,  G01N21/82 ,  G01N33/53 ,  G01N21/02 ,  G01N31/02 ,  G01N33/16

CPC分类号： G01N21/82 ,  G01N33/53 ,  Y10S436/805 ,  Y10S436/808 ,  Y10S436/815

摘要： A method for the quantitative determination of antigenic substance without any confirmation test which comprises adding to a sample to be assayed an exogenous antigenic substance in a certain amount, combining the resulting mixture with an antibody specific to the antigenic substance to cause antigen-antibody reaction and measuring the amount of the precipitates formed, and a diagnostic kit for practicing the method are disclosed.

摘要翻译： 一种定量测定抗原物质的方法，无任何确认试验，其中包括加入一定量的外源抗原物质样品，将所得混合物与抗原物质特异性抗体结合，引起抗原抗体反应， 测量形成的沉淀物的量，并且公开了用于实施该方法的诊断试剂盒。