公开(公告)号：US07846662B2

公开(公告)日：2010-12-07

申请号：US11662795

申请日：2005-09-14

Applicant: Tsutomu Takeuchi ,  Kensei Tsuzuka ,  Keiko Yoshimoto

Inventor： Tsutomu Takeuchi ,  Kensei Tsuzuka ,  Keiko Yoshimoto

IPC: C12Q1/68

CPC classification number: C07K16/2875 ,  A61K31/341 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/6863 ,  G01N2500/00

Abstract: A method of screening a novel BAFF suppressor or inhibitor. More specifically speaking, a method which comprises adding a combination of TPA with ionomycin and/or an anti-CD3 antibody to a cultured human cell to thereby induce the production of BAFF by the cell; a method of screening a substance capable of suppressing the expression or activity of BAFF which comprises adding a test substance to a BAFF-production system prepared by adding a combination of TPA with ionomycin and/or an anti-CD3 antibody to a cultured human cell and measuring the expression amount and/or the activity of BAFF in the BAFF-production system; and a BAFF production inducer for a BAFF-producing cell which contains a combination of TPA with ionomycin and/or an anti-CD3 antibody.

Abstract translation: 筛选新型BAFF抑制剂或抑制剂的方法。 更具体地说，包括将TPA与离子霉素和/或抗CD3抗体的组合加入到培养的人细胞中，从而诱导细胞产生BAFF的方法; 筛选能够抑制BAFF的表达或活性的物质的方法，其包括向经培养的人细胞添加TPA与离子霉素和/或抗CD3抗体的组合而制备的BAFF-生产系统中的测试物质，以及 测量BAFF在BAFF生产系统中的表达量和/或活性; 以及含有TPA与离子霉素和/或抗CD3抗体的组合的BAFF产生细胞的BAFF产生诱导剂。