公开(公告)号：US20130065278A1

公开(公告)日：2013-03-14

申请号：US13634519

申请日：2010-10-27

Applicant: Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

Inventor： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

IPC: C07K19/00 ,  C12N15/62 ,  C12P21/02 ,  C12N9/96

CPC classification number: C07K14/43518 ,  C12N15/62

Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

Abstract translation: 产生所需非宿主蛋白或多肽的方法包括以下步骤：在合适的宿主中表达融合蛋白，获得含有融合蛋白的混合物，以及任选地分离融合蛋白。 融合蛋白质包含衍生自蜘蛛丝蛋白的N末端（NT）片段的至少一种溶解度增强部分。 其还包含至少一个作为期望的非宿主蛋白或多肽的部分。 每个溶解度增强部分直接或间接连接到所需的蛋白质或多肽部分。

公开(公告)号：US09644012B2

公开(公告)日：2017-05-09

申请号：US13634519

申请日：2010-10-27

Applicant: Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

Inventor： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

IPC: C07K14/435 ,  C12N15/62

CPC classification number: C07K14/43518 ,  C12N15/62

Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

公开(公告)号：US08642734B2

公开(公告)日：2014-02-04

申请号：US13264938

申请日：2010-04-21

Applicant: Jan Johansson ,  My Hedhammar ,  Anna Rising ,  Kerstin Nordling

Inventor： Jan Johansson ,  My Hedhammar ,  Anna Rising ,  Kerstin Nordling

IPC: A61K38/17

CPC classification number: C07K14/43518

Abstract: A method of producing polymers of an isolated spider silk protein involves providing a solution of said spider silk protein in a liquid medium at pH 6.4 or higher and/or an ion composition that prevents polymerization of the spider silk protein. The properties of the liquid medium are adjusted to a pH of 6.3 or lower and an ion composition that allows polymerization of the spider silk protein. The spider silk protein is allowed to form polymers in the liquid medium, and the resulting spider silk protein polymers are isolated from the liquid medium. The resulting polymers are useful as fibers, films, foams, nets or meshes.

Abstract translation: 制备分离的蜘蛛丝蛋白质的聚合物的方法包括提供所述蜘蛛丝蛋白质在pH6.4或更高的液体培养基中的溶液和/或防止蜘蛛丝蛋白聚合的离子组合物。 将液体介质的性质调节至6.3或更低的pH，以及允许蜘蛛丝蛋白质聚合的离子组合物。 允许蜘蛛丝蛋白在液体培养基中形成聚合物，并且从液体培养基中分离得到的蜘蛛丝蛋白聚合物。 所得聚合物可用作纤维，薄膜，泡沫，网或网。

公开(公告)号：US20120041177A1

公开(公告)日：2012-02-16

申请号：US13264938

申请日：2010-04-21

Applicant: Jan Johansson ,  My Hedhammar ,  Anna Rising ,  Kerstin Nordling

Inventor： Jan Johansson ,  My Hedhammar ,  Anna Rising ,  Kerstin Nordling

IPC: C07K14/435 ,  C12P21/00 ,  C07K1/14

CPC classification number: C07K14/43518

Abstract: A method of producing polymers of an isolated spider silk protein involves providing a solution of said spider silk protein in a liquid medium at pH 6.4 or higher and/or an ion composition that prevents polymerisation of the spider silk protein. The properties of the liquid medium are adjusted to a pH of 6.3 or lower and an ion composition that allows polymerisation of the spider silk protein. The spider silk protein is allowed to form polymers in the liquid medium, and the resulting spider silk protein polymers are isolated from the liquid medium. The resulting polymers are useful as fibers, films, foams, nets or meshes.

Abstract translation: 制备分离的蜘蛛丝蛋白的聚合物的方法包括提供所述蜘蛛丝蛋白在pH6.4或更高的液体培养基中的溶液和/或防止蜘蛛丝蛋白聚合的离子组合物。 将液体介质的性质调节至6.3或更低的pH，以及允许蜘蛛丝蛋白质聚合的离子组合物。 允许蜘蛛丝蛋白在液体培养基中形成聚合物，并且从液体培养基中分离得到的蜘蛛丝蛋白聚合物。 所得聚合物可用作纤维，薄膜，泡沫，网或网。