公开(公告)号：US09522170B2

公开(公告)日：2016-12-20

申请号：US14009360

申请日：2012-03-30

申请人： Jan Johansson ,  Jenny Presto

发明人： Jan Johansson ,  Jenny Presto

IPC分类号： G01N33/53 ,  G01N33/567 ,  A61K38/00 ,  A61K49/00 ,  A61K38/17 ,  G01N33/68 ,  C07K14/47 ,  G01N33/50

CPC分类号： A61K38/1709 ,  A61K38/00 ,  C07K14/47 ,  G01N33/5058 ,  G01N33/6896 ,  G01N2500/02 ,  G01N2800/12 ,  G01N2800/2821

摘要： A method involves screening a candidate compound for activity in the treatment of a condition associated with formation of amyloid protein fibrils in a mammal, such as Alzheimer's disease. It is determined whether the trimer/monomer ratio of a chaperone protein is decreased in the presence of the candidate compound. The chaperone protein is or has a high identity to the Brichos domains of Bri2, Bri3 or proSP-C from human. Monomers of the chaperone proteins and/or compounds that promote formation of these monomers are useful for medical treatment of the condition.

摘要翻译： 一种方法包括筛选候选化合物用于治疗与哺乳动物如阿尔茨海默病中淀粉样蛋白原纤维形成有关的病症的活性。 在候选化合物的存在下，确定伴侣蛋白的三聚体/单体比是否降低。 伴侣蛋白与来自人的Bri2，Bri3或proSP-C的Brichos结构域具有或具有高同一性。 促进这些单体形成的分子伴侣蛋白和/或化合物的单体可用于治疗该病症。

公开(公告)号：US09115204B2

公开(公告)日：2015-08-25

申请号：US13880628

申请日：2011-10-25

申请人： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

发明人： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC分类号： C07K14/435 ,  A61K47/48 ,  B01D15/38 ,  B01J20/281 ,  C07K16/06

CPC分类号： C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

摘要： A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

摘要翻译： 提供能够与有机靶相互选择性的蛋白质结构。 蛋白质结构是包含能够与有机靶选择性相互作用的重组融合蛋白作为重复结构单元的聚合物。 融合蛋白包含部分B，REP和CT，以及任选的NT。 B是超过30个氨基酸残基的非宿主部分，其提供与有机靶的选择性相互作用的能力。 REP是70至300个氨基酸残基的部分，并衍生自蜘蛛丝蛋白的重复片段。 CT是70至120个氨基酸残基的部分，衍生自蜘蛛丝蛋白的C末端片段。 NT是100至160个氨基酸残基的任选部分，并衍生自蜘蛛丝蛋白的N-末端片段。 其融合蛋白质和蛋白质结构可用作亲和介质和细胞支架材料。

公开(公告)号：US08987192B2

公开(公告)日：2015-03-24

申请号：US12519116

申请日：2007-12-13

申请人： John K. Bielicki ,  Jan Johansson

发明人： John K. Bielicki ,  Jan Johansson

IPC分类号： A61K38/00 ,  A61K38/16 ,  A61K45/00 ,  A61K47/24 ,  A61K49/00 ,  A61K51/00 ,  A61K9/127 ,  C07K14/00 ,  C07K14/47 ,  C07K14/705 ,  A61P3/06 ,  A61P9/00 ,  C07K14/775

CPC分类号： C07K14/775 ,  A61K38/00 ,  A61K38/16 ,  C07K14/4703

摘要： The present invention provides a family of non-naturally occurring polypeptides having cholesterol efflux activity that parallels that of full-length apolipoproteins (e.g., Apo AI and Apo E), and having high selectivity for ABAC1 that parallels that of full-length apolipoproteins. The invention also provides compositions comprising such polypeptides, methods of identifying, screening and synthesizing such polypeptides, and methods of treating, preventing or diagnosing diseases and disorders associated with dyslipidemia, hypercholesterolemia and inflammation.

摘要翻译： 本发明提供了具有与全长载脂蛋白（例如Apo AI和Apo E）平行的胆固醇流出活性的非天然存在的多肽的家族，并且对于与全长载脂蛋白的ABAC1平行的ABAC1具有高选择性。 本发明还提供包含这些多肽的组合物，鉴定，筛选和合成这些多肽的方法，以及治疗，预防或诊断与血脂异常，高胆固醇血症和炎症相关的疾病和病症的方法。

公开(公告)号：US08785390B2

公开(公告)日：2014-07-22

申请号：US13145096

申请日：2010-01-29

申请人： Jan Johansson

发明人： Jan Johansson

IPC分类号： A61K38/17 ,  C07K14/785

CPC分类号： A61K38/395 ,  A61K38/00 ,  A61K38/1709 ,  C07K14/785

摘要： An isolated protein selected from the group consisting of (i) proteins comprising an amino acid sequence having at least 70% identity to the C-terminal domain of lung surfactant protein C precursor (CTproSP-C, “CTC”) from a mammal; and (ii) proteins comprising an amino acid sequence having at least 70% identity to the Brichos domain of CTproSP-C from a mammal, is disclosed for treatment of Alzheimer's disease in a mammal, including man.

摘要翻译： 选自以下的分离蛋白质：（i）包含与来自哺乳动物的肺表面活性蛋白C前体（CTproSP-C，“CTC”）的C-末端结构域具有至少70％同一性的氨基酸序列的蛋白质; 公开了包含与来自哺乳动物的CTproSP-C的Brichos结构域具有至少70％同一性的氨基酸序列的蛋白质，包括人在内的哺乳动物治疗阿尔茨海默氏病。

公开(公告)号：US20140030274A1

公开(公告)日：2014-01-30

申请号：US14009360

申请日：2012-03-30

申请人： Jan Johansson ,  Jenny Presto

发明人： Jan Johansson ,  Jenny Presto

IPC分类号： A61K38/17 ,  G01N33/50

CPC分类号： A61K38/1709 ,  A61K38/00 ,  C07K14/47 ,  G01N33/5058 ,  G01N33/6896 ,  G01N2500/02 ,  G01N2800/12 ,  G01N2800/2821

摘要： A method involves screening a candidate compound for activity in the treatment of a condition associated with formation of amyloid protein fibrils in a mammal, such as Alzheimer's disease. It is determined whether the trimer/monomer ratio of a chaperone protein is decreased in the presence of the candidate compound. The chaperone protein is or has a high identity to the Brichos domains of Bri2, Bri3 or proSP-C from human. Monomers of the chaperone proteins and/or compounds that promote formation of these monomers are useful for medical treatment of the condition.

摘要翻译： 一种方法包括筛选候选化合物用于治疗与哺乳动物如阿尔茨海默病中淀粉样蛋白原纤维形成有关的病症的活性。 在候选化合物的存在下，确定伴侣蛋白的三聚体/单体比是否降低。 伴侣蛋白与来自人的Bri2，Bri3或proSP-C的Brichos结构域具有或具有高同一性。 促进这些单体形成的分子伴侣蛋白和/或化合物的单体可用于治疗该病症。

公开(公告)号：US08618255B2

公开(公告)日：2013-12-31

申请号：US13598119

申请日：2012-08-29

申请人： Jan Johansson ,  Göran Hjäm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engström ,  My Hedhammar

发明人： Jan Johansson ,  Göran Hjäm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engström ,  My Hedhammar

IPC分类号： C07K1/00 ,  C07K14/00

CPC分类号： C07K14/43518 ,  A61K35/12 ,  A61K47/42 ,  D01F4/00

摘要： The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

摘要翻译： 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式REP-CT定义。 REP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。

公开(公告)号：US20130316376A1

公开(公告)日：2013-11-28

申请号：US13880628

申请日：2011-10-25

申请人： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

发明人： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC分类号： C07K14/435

CPC分类号： C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

摘要： A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

公开(公告)号：US20130176841A1

公开(公告)日：2013-07-11

申请号：US13809409

申请日：2010-07-09

申请人： Aijun Cao ,  Jan Johansson ,  Xiaoming Su ,  Yujie Li

发明人： Aijun Cao ,  Jan Johansson ,  Xiaoming Su ,  Yujie Li

IPC分类号： H04W72/04

CPC分类号： H04W72/0413 ,  H04L1/0026 ,  H04L1/1671 ,  H04L5/0007 ,  H04L5/0048 ,  H04L27/2613

摘要： The present document discloses an apparatus for receiving Physical Uplink Control Channel (PUCCH) in Long Term Evolution (LTE) system, comprising: blocks of system FFT performing system FFT on data from a plurality of antennas, blocks of PUCCH RB processing units receiving the allocated logic resource index table and the local base sequence and output of the blocks of system FFT, and generating DMRS despread outputs and data values; a block of SR detection for receiving the data values and the DMRS despread outputs, and for generating SR detection results; blocks of user data extraction receiving data values and DMRS despread outputs, and performing user data extraction; blocks of PUCCH user processing units receiving an orthogonal sequence index, the data values and an output from blocks of user data extraction, and generating a ACK/NAK/CQI signal for a concerned UE.

摘要翻译： 本文件公开了一种在长期演进（LTE）系统中接收物理上行链路控制信道（PUCCH）的装置，包括：对来自多个天线的数据执行系统FFT的系统FFT块，接收所分配的PUCCH RB处理单元的块 逻辑资源索引表和系统FFT的本地基序列和输出，并产生DMRS解扩输出和数据值; 用于接收数据值和DMRS解扩输出的SR检测块，以及用于产生SR检测结果; 接收数据值的用户数据提取块和DMRS解扩输出，并执行用户数据提取; 接收正交序列索引的PUCCH用户处理单元的块，数据值和来自用户数据提取的块的输出，以及为相关的UE生成ACK / NAK / CQI信号。

公开(公告)号：US20130172262A1

公开(公告)日：2013-07-04

申请号：US13805574

申请日：2010-06-24

申请人： Jan Johansson

发明人： Jan Johansson

IPC分类号： C07K14/47

CPC分类号： C07K14/47 ,  A61K9/0019 ,  A61K38/00 ,  C07K14/4711

摘要： An isolated protein is provided for use in treatment of a condition selected from the group consisting of Alzheimer's disease, familial Danish dementia and familial British dementia in a mammal, including man. The isolated protein is selected from the group consisting of proteins comprising an amino acid sequence having at least 70% identity to residues 90-236 of Bri2 from human; and proteins comprising an amino acid sequence having at least 70% identity to any one of the Brichos domains of Bri2 from human, chimpanzee, bovine, pig, mouse and rat.

摘要翻译： 提供分离的蛋白质用于治疗哺乳动物（包括人）中从阿尔茨海默病，家族性丹麦痴呆和家族性英国痴呆中选择的病症。 分离的蛋白质选自包含与来自人的Bri2的残基90-236具有至少70％同一性的氨基酸序列的蛋白质; 和包含与来自人，黑猩猩，牛，猪，小鼠和大鼠的Bri2的任何一个Brichos结构域具有至少70％同一性的氨基酸序列的蛋白质。

公开(公告)号：US20130065278A1

公开(公告)日：2013-03-14

申请号：US13634519

申请日：2010-10-27

申请人： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

发明人： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

IPC分类号： C07K19/00 ,  C12N15/62 ,  C12P21/02 ,  C12N9/96

CPC分类号： C07K14/43518 ,  C12N15/62

摘要： A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

摘要翻译： 产生所需非宿主蛋白或多肽的方法包括以下步骤：在合适的宿主中表达融合蛋白，获得含有融合蛋白的混合物，以及任选地分离融合蛋白。 融合蛋白质包含衍生自蜘蛛丝蛋白的N末端（NT）片段的至少一种溶解度增强部分。 其还包含至少一个作为期望的非宿主蛋白或多肽的部分。 每个溶解度增强部分直接或间接连接到所需的蛋白质或多肽部分。