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公开(公告)号:US08158772B2
公开(公告)日:2012-04-17
申请号:US10826119
申请日:2004-04-16
申请人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
发明人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
CPC分类号: C12Q1/6888 , C12Q1/6881
摘要: Methods for identifying animal species involving amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, the animal-specific DNA sequences derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and detecting the amplified DNA fragment.
摘要翻译: 用于鉴定通过使用样品中的DNA作为模板并以动物特异性DNA序列作为引物对的PCR扩增DNA片段的动物物种的方法,来源于ATP合酶亚基8基因或近端区域的动物特异性DNA序列 线粒体基因组; 并检测扩增的DNA片段。
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公开(公告)号:US20050233334A1
公开(公告)日:2005-10-20
申请号:US10826119
申请日:2004-04-16
申请人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
发明人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
CPC分类号: C12Q1/6888 , C12Q1/6881
摘要: The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.
摘要翻译: 本发明提供了一种用于鉴定动物种类的方法,所述方法包括使用样品中的DNA作为模板并以动物特异性DNA序列作为引物对通过PCR扩增DNA片段的步骤,其中所述动物特异性DNA序列 衍生自ATP合成酶亚基8基因或线粒体基因组近端的区域; 以及检测扩增的DNA片段的步骤。
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公开(公告)号:US20140234849A1
公开(公告)日:2014-08-21
申请号:US14185070
申请日:2014-02-20
申请人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
发明人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6888 , C12Q1/6881
摘要: The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.
摘要翻译: 本发明提供了一种用于鉴定动物种类的方法,所述方法包括使用样品中的DNA作为模板并以动物特异性DNA序列作为引物对通过PCR扩增DNA片段的步骤,其中所述动物特异性DNA序列 衍生自ATP合成酶亚基8基因或线粒体基因组近端的区域; 以及检测扩增的DNA片段的步骤。
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公开(公告)号:US20120270223A1
公开(公告)日:2012-10-25
申请号:US13417692
申请日:2012-03-12
申请人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
发明人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6888 , C12Q1/6881
摘要: The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.
摘要翻译: 本发明提供了一种用于鉴定动物种类的方法,所述方法包括使用样品中的DNA作为模板并以动物特异性DNA序列作为引物对通过PCR扩增DNA片段的步骤,其中所述动物特异性DNA序列 衍生自ATP合成酶亚基8基因或线粒体基因组近端的区域; 以及检测扩增的DNA片段的步骤。
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公开(公告)号:US07723569B2
公开(公告)日:2010-05-25
申请号:US11119351
申请日:2005-04-29
CPC分类号: C12N9/1085 , C12N15/8243
摘要: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.
摘要翻译: 提供了表达大量泛醌-10的植物和使用植物生产泛醌-10的方法。 提供了由植物或方法生产的含有泛醌-10的膳食补充剂,食品和食品添加剂。 通过使用表达盒转化来产生植物,其中十聚丙烯酰二磷酸合成酶基因与线粒体靶向序列可操作地连接。
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公开(公告)号:US20100184078A1
公开(公告)日:2010-07-22
申请号:US12707828
申请日:2010-02-18
申请人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
发明人: Toyoko Kusama , Koichi Kadowaki , Tetsuya Nomura
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6888 , C12Q1/6881
摘要: The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.
摘要翻译: 本发明提供了一种用于鉴定动物种类的方法,所述方法包括使用样品中的DNA作为模板并以动物特异性DNA序列作为引物对通过PCR扩增DNA片段的步骤,其中所述动物特异性DNA序列 衍生自ATP合成酶亚基8基因或线粒体基因组近端的区域; 以及检测扩增的DNA片段的步骤。
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公开(公告)号:US20060010519A1
公开(公告)日:2006-01-12
申请号:US11119351
申请日:2005-04-29
CPC分类号: C12N9/1085 , C12N15/8243
摘要: A plant which expresses a large amount of ubiquinone-10 and a method for producing ubiquinone-10 using the plant are provided. A dietary supplement, a food and a food additive which contains ubiquinone-10 produced by the plant or the method are provided. The plant is produced by transformation using an expression cassette in which a decaprenyl diphosphate synthase gene is operatively linked with a mitochondrial targeting sequence.
摘要翻译: 提供了表达大量泛醌-10的植物和使用植物生产泛醌-10的方法。 提供了由植物或方法生产的含有泛醌-10的膳食补充剂,食品和食品添加剂。 通过使用表达盒转化来产生植物,其中十聚丙烯酰二磷酸合成酶基因与线粒体靶向序列可操作地连接。
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