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公开(公告)号：US20120329069A1
公开(公告)日：2012-12-27
申请号：US13572239
申请日：2012-08-10
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
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公开(公告)号：US07960517B2
公开(公告)日：2011-06-14
申请号：US12263002
申请日：2008-10-31
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
摘要翻译： 本发明提供了鉴定抗体的位置的方法，该方法可以在不显着降低抗体结合活性的情况下进行修饰。 在许多实施方案中，该方法包括通过将其氨基酸序列与多个与亲本抗体相同的抗原结合的多种相关抗体的氨基酸序列进行比较来鉴定亲本抗体中的可取代位置。 可取代位置的氨基酸可以代替不同的氨基酸而不显着影响抗体的活性。 可以使用本发明的方法来改变CDR的氨基酸序列，而不显着降低抗体的抗体在人源化方法或其它抗体工程方法中的亲和力。 本发明可用于各种治疗，诊断和研究应用。
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公开(公告)号：US20090155824A1
公开(公告)日：2009-06-18
申请号：US12263002
申请日：2008-10-31
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
摘要翻译： 本发明提供了鉴定抗体的位置的方法，该方法可以在不显着降低抗体结合活性的情况下进行修饰。 在许多实施方案中，该方法包括通过将其氨基酸序列与多个与亲本抗体相同的抗原结合的多种相关抗体的氨基酸序列进行比较来鉴定亲本抗体中的可取代位置。 可取代位置的氨基酸可以代替不同的氨基酸而不显着影响抗体的活性。 可以使用本发明的方法来改变CDR的氨基酸序列，而不显着降低抗体的抗体在人源化方法或其它抗体工程方法中的亲和力。 本发明可用于各种治疗，诊断和研究应用。
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公开(公告)号：US07462697B2
公开(公告)日：2008-12-09
申请号：US10984473
申请日：2004-11-08
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
摘要翻译： 本发明提供了鉴定抗体的位置的方法，该方法可以在不显着降低抗体结合活性的情况下进行修饰。 在许多实施方案中，该方法包括通过将其氨基酸序列与多个与亲本抗体相同的抗原结合的多种相关抗体的氨基酸序列进行比较来鉴定亲本抗体中的可取代位置。 可取代位置的氨基酸可以代替不同的氨基酸而不显着影响抗体的活性。 可以使用本发明的方法来改变CDR的氨基酸序列，而不显着降低抗体的抗体在人源化方法或其它抗体工程方法中的亲和力。 本发明可用于各种治疗，诊断和研究应用。
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公开(公告)号：US08404816B2
公开(公告)日：2013-03-26
申请号：US13100089
申请日：2011-05-03
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
摘要翻译： 本发明提供了鉴定抗体的位置的方法，该方法可以在不显着降低抗体结合活性的情况下进行修饰。 在许多实施方案中，该方法包括通过将其氨基酸序列与多个与亲本抗体相同的抗原结合的多种相关抗体的氨基酸序列进行比较来鉴定亲本抗体中的可取代位置。 可取代位置的氨基酸可以代替不同的氨基酸而不显着影响抗体的活性。 可以使用本发明的方法来改变CDR的氨基酸序列，而不显着降低抗体的抗体在人源化方法或其它抗体工程方法中的亲和力。 本发明可用于各种治疗，诊断和研究应用。
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公开(公告)号：US20120003671A1
公开(公告)日：2012-01-05
申请号：US13100089
申请日：2011-05-03
IPC分类号： G01N33/566 , C07K16/00 , C12P21/00
CPC分类号： G01N33/577 , C07K16/241 , C07K16/467
摘要： The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications.
摘要翻译： 本发明提供了鉴定抗体的位置的方法，该方法可以在不显着降低抗体结合活性的情况下进行修饰。 在许多实施方案中，该方法包括通过将其氨基酸序列与多个与亲本抗体相同的抗原结合的多种相关抗体的氨基酸序列进行比较来鉴定亲本抗体中的可取代位置。 可取代位置的氨基酸可以代替不同的氨基酸而不显着影响抗体的活性。 可以使用本发明的方法来改变CDR的氨基酸序列，而不显着降低抗体的抗体在人源化方法或其它抗体工程方法中的亲和力。 本发明可用于各种治疗，诊断和研究应用。
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