公开(公告)号：US20100216657A1

公开(公告)日：2010-08-26

申请号：US12301099

申请日：2007-05-16

申请人： Kyle Wisdom Hukari ,  Jason Andrew Appleton West ,  Dustin Li ,  Brent Coleman Satterfield ,  Lars L. Majlof

发明人： Kyle Wisdom Hukari ,  Jason Andrew Appleton West ,  Dustin Li ,  Brent Coleman Satterfield ,  Lars L. Majlof

IPC分类号： C40B30/04 ,  C40B40/06

CPC分类号： G01N21/6428 ,  B01L3/0275 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2300/0636 ,  G01N21/6456 ,  G01N2021/6482 ,  G01N2035/00158

摘要： Provided herein are biologic sample preparation and analysis systems that are rapid, portable, robust detection system for multiplexed detection of bio-threats, and which can be ruggedized to operate in harsh environments. A new method of detection called Combinatorial Probe Analysis (CPA), which provides an exponential increase in detection reliability, has been incorporated into these systems. This type of analysis greatly reduces false positives and false negatives; in addition it is reusable and eliminates special storage requirements for reagents. Specific technical advancements in the optimization of hybridization assays for nucleic acid detection on porous polymer monoliths (PPM) are also disclosed. Performing rapid and complete solubilization of viruses, vegetative bacteria and bacterial spores with an ultra high temperature solubilization protocol is also described. The systems provided herein provides the ability to perform rapid highly multiplexed analysis of a variety of bioagents, including bacteria viruses, and protein biotoxins. The systems and assays described herein are perform completely automated sample preparation and analysis, in a time frame of five minutes or less. The assay is simple in design allowing users in personal protective equipment to easily operate the system. The disclosed systems are robust, simple to use, and address the goals of the first responder community.

摘要翻译： 本文提供了生物样品制备和分析系统，其是用于多重检测生物威胁的快速，便携，鲁棒的检测系统，并且其可以坚固地在恶劣环境中操作。 称为组合探测分析（CPA）的一种新的检测方法，其检测可靠性提高了指数增长，已被纳入这些系统。 这种分析大大减少了假阳性和假阴性; 此外，它是可重复使用的，并且消除了对试剂的特殊储存要求。 还公开了在多孔聚合物整料（PPM）上的核酸检测的杂交测定的优化中的具体技术进步。 还描述了用超高温溶解方案快速和完全地溶解病毒，营养细菌和细菌孢子。 本文提供的系统提供对多种生物代谢物进行快速高度多重分析的能力，包括细菌病毒和蛋白质生物毒素。 本文所述的系统和测定在五分钟或更短的时间内执行完全自动化的样品制备和分析。 该测定设计简单，允许个人防护设备中的用户轻松操作系统。 所公开的系统是健壮的，易于使用的，并且解决了第一响应者社区的目标。