公开(公告)号：US20240241059A1

公开(公告)日：2024-07-18

申请号：US18579845

申请日：2022-07-29

Applicant: Nicoya Lifesciences Inc.

Inventor： Seray CICEK ,  Yuxiu GUO ,  Katariina Hanna Zakaaria Sepp JAENES ,  Lucas ROBINSON ,  Afifa SALEEM

IPC: G01N21/77 ,  G01N33/543 ,  G01N33/569 ,  G01N35/00 ,  G01N35/10

CPC classification number: G01N21/77 ,  G01N33/54387 ,  G01N33/56983 ,  G01N35/00029 ,  G01N35/00732 ,  G01N35/1095 ,  G01N2021/7786 ,  G01N2035/00158

Abstract: There are provided systems and methods for molecular diagnostic reaction testing. The method including: receiving a patient sample in a collection device: providing the sample to a microfluidic cartridge via capillary action: inserting the microfluidic cartridge into a diagnostic device: capturing information associated with the sample: performing thermal lysis and inactivation on the sample in the microfluidic cartridge: performing sample mixing with a freeze-dried master mix by passing the sample through microfluidic channels in the microfluidic cartridge that enables mixing of the samples with the freeze-dried master mix: perform multiplex detection of different targets by passing the mixed sample into detection chambers containing probes: capturing images of the mixed samples in the detection chambers: and outputting the images.

公开(公告)号：US20240060872A1

公开(公告)日：2024-02-22

申请号：US18384323

申请日：2023-10-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique

IPC: G01N15/14 ,  C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  G01N1/28 ,  G01N1/40 ,  G01N1/20

CPC classification number: G01N15/1484 ,  C12M47/04 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/021 ,  G01N1/28 ,  G01N1/405 ,  B01L3/502746 ,  G01N1/20 ,  G01N1/40 ,  B01L2300/0636 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  G01N1/4077 ,  G01N2015/149 ,  G01N2035/00158

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

公开(公告)号：US11815512B2

公开(公告)日：2023-11-14

申请号：US16925224

申请日：2020-07-09

Applicant: Vibrant Holdings, LLC

Inventor： John J. Rajasekaran ,  Vasanth Jayaraman ,  Tianhao Wang ,  Kang Bei ,  Hari Krishnan Krishnamurthy

IPC: G01N33/543 ,  G01N35/00 ,  C40B40/10 ,  C40B40/06 ,  G16B20/00 ,  G01N35/10

CPC classification number: G01N33/54366 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54386 ,  G01N35/00029 ,  G01N35/00069 ,  G01N35/0099 ,  G16B20/00 ,  G01N2035/00158 ,  G01N2035/00168 ,  G01N2035/00326 ,  G01N2035/1034

Abstract: Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and methods of assuring a uniformly high quality of a microarray of features that are attached to a surface of the microarray at positionally-defined locations.

公开(公告)号：US11674956B2

公开(公告)日：2023-06-13

申请号：US17839160

申请日：2022-06-13

Applicant: Vibrant Holdings, LLC

Inventor： John J. Rajasekaran ,  Vasanth Jayaraman ,  Tianhao Wang ,  Kang Bei ,  Hari Krishnan Krishnamurthy

IPC: G01N33/543 ,  G01N35/00 ,  C40B40/10 ,  C40B40/06 ,  G16B20/00 ,  G01N35/10

CPC classification number: G01N33/54366 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54386 ,  G01N35/00029 ,  G01N35/00069 ,  G01N35/0099 ,  G16B20/00 ,  G01N2035/00158 ,  G01N2035/00168 ,  G01N2035/00326 ,  G01N2035/1034

Abstract: Disclosed herein are methods for collecting data from chip arrays and for piecewise real-time scanning and stitching of said data. Such arrays can comprise a plurality of microarrays, each microarray comprising biomolecular features that are attached to a surface of the microarray.

公开(公告)号：US20190216363A1

公开(公告)日：2019-07-18

申请号：US16155480

申请日：2018-10-09

Applicant: Theranos IP Company, LLC

Inventor： Elizabeth A. Holmes ,  Shaunak Roy ,  John Howard ,  Chengwang Wang

IPC: A61B5/07 ,  A61B5/151 ,  A61B5/15 ,  A61B5/00 ,  A61B5/157 ,  A61B5/145 ,  B01L3/00 ,  A61M37/00 ,  A61B5/1477 ,  A61B5/1473 ,  A61B5/1455

CPC classification number: A61B5/073 ,  A61B5/0024 ,  A61B5/0031 ,  A61B5/1411 ,  A61B5/145 ,  A61B5/14514 ,  A61B5/14532 ,  A61B5/14542 ,  A61B5/14546 ,  A61B5/1455 ,  A61B5/14556 ,  A61B5/1473 ,  A61B5/1477 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150221 ,  A61B5/150229 ,  A61B5/150251 ,  A61B5/150282 ,  A61B5/150343 ,  A61B5/150854 ,  A61B5/150862 ,  A61B5/15087 ,  A61B5/150969 ,  A61B5/150984 ,  A61B5/15107 ,  A61B5/15117 ,  A61B5/15119 ,  A61B5/15121 ,  A61B5/15123 ,  A61B5/15125 ,  A61B5/1519 ,  A61B5/157 ,  A61B5/417 ,  A61B5/4238 ,  A61B5/4839 ,  A61B5/4848 ,  A61B5/6833 ,  A61B5/685 ,  A61B5/6865 ,  A61B5/7282 ,  A61B2560/0412 ,  A61M5/14276 ,  A61M37/0015 ,  A61M2005/14513 ,  A61M2005/1726 ,  A61M2037/0023 ,  A61M2205/0244 ,  A61M2205/3569 ,  A61M2205/3592 ,  A61M2205/50 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/10 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0472 ,  B01L2400/0487 ,  G01N2035/00158

Abstract: The invention relates to an ingestible, implantable or wearable medical device comprising a microarray which comprises a bioactive agent capable of interacting with a disease marker biological analyte; a reservoir which comprises at least one therapeutic agent and is capable of releasing the therapeutic agent(s) from the medical device; and a plurality of microchips comprising a microarray scanning device capable of obtaining physical parameter data of an interaction between the disease marker biological analyte with the bioactive agent; a biometric recognition device capable of comparing the physical parameter data with an analyte interaction profile; optionally a therapeutic agent releasing device capable of controlling release of the therapeutic agent from the reservoirs; an interface device capable of facilitating communications between the microarray scanning device, biometric recognition device and the therapeutic agent releasing device; and an energy source to power the medical device. Specifically, the invention relates to a medical device capable of detecting an analyte in a bodily fluid comprising at least one microneedle capable of obtaining a sample of a bodily fluid, a first microchannel through which the sample flows and is in fluid communication with the at least one microneedle, a second microchannel in fluid communication with the first microchannel, through which a buffer flows, wherein the second channel comprises a microarray with a bioactive agent, a microarray scanning device to detect an interaction between the bioactive agent and the analyte in the bodily fluid; and an interface device.

公开(公告)号：US20180236446A1

公开(公告)日：2018-08-23

申请号：US15898097

申请日：2018-02-15

Applicant: Cambsolv Limited

Inventor： Hugh Malkin ,  Nicholas James Hastings ,  Graham Scott Gutsell ,  Ian Morgan George

IPC: B01L3/00 ,  G01N35/00

CPC classification number: B01L3/502715 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2200/0605 ,  B01L2200/0631 ,  B01L2200/16 ,  B01L2300/049 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2400/049 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N35/0099 ,  G01N2035/00158 ,  G01N2035/0425

Abstract: Described is a modular microfluidic device (MMD) for producing an analytic composition from a biological fluid sample, said device comprising a reagent module (RM) comprising a reagent reservoir containing a reagent and an eluent reservoir containing an eluent, said reagent and eluent reservoirs being coupled to one or more RM microchannels; and a sample preparation module (SPM) comprising a SPM microchannel adapted to couple with the RM microchannel whereby fluid continuity between SPM and RM microchannels is produced on coupling, and: (i) a sample inlet for receiving said biological fluid sample; (ii) an outlet for delivering said analyte composition; (iii) a sensor component; (iv) a mixing chamber; (v) a metering chamber; (vi) an eluent chamber; (vii) a valve; and (viii) a solid phase extraction element (SPE); wherein said metering chamber is of fixed volume and shape and substantially ellipsoidal, the metering chamber having an inlet and an outlet, said inlet and/or outlet being in fluid communication with one or more microchannel(s) of the MMD.

公开(公告)号：US20180188276A1

公开(公告)日：2018-07-05

申请号：US15853332

申请日：2017-12-22

Applicant: xCella Biosciences, Inc.

Inventor： Bob CHEN

IPC: G01N35/10 ,  G01N21/75 ,  C12N15/10 ,  C40B60/02 ,  B01J19/00 ,  C12Q1/6851 ,  B01L3/00

CPC classification number: G01N35/10 ,  B01J19/0046 ,  B01J2219/00306 ,  B01J2219/00509 ,  B01J2219/00511 ,  B01J2219/00524 ,  B01J2219/00587 ,  B01J2219/00599 ,  B01J2219/00684 ,  B01J2219/00722 ,  B01J2219/00869 ,  B01J2219/0097 ,  B01L3/502715 ,  B01L3/50857 ,  B01L9/523 ,  B01L2200/0668 ,  B01L2200/142 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0893 ,  C12N15/1003 ,  C12Q1/6851 ,  C12Q2531/113 ,  C40B60/02 ,  G01N15/1463 ,  G01N15/1475 ,  G01N15/1484 ,  G01N21/6428 ,  G01N21/75 ,  G01N33/54386 ,  G01N2015/1006 ,  G01N2021/0346 ,  G01N2021/6439 ,  G01N2021/755 ,  G01N2021/7769 ,  G01N2035/00158 ,  G01N2035/1039 ,  G01N2035/1053

Abstract: Multi-stage sample-recovery systems, including automated 2-stage and 3-stage sample-recovery systems, are provided. Such systems enable the rapid screening and recovery of samples, including viable cell-based samples, from high-throughput screening systems, including systems utilizing large-scale arrays of microcapillaries. In specific screening systems, each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be identified and recovered using the multi-stage systems disclosed herein.

公开(公告)号：US20180179481A1

公开(公告)日：2018-06-28

申请号：US15824081

申请日：2017-11-28

Applicant: TOKYO OHKA KOGYO CO., LTD. ,  NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Takashi FUJIMOTO ,  Hidenori MIYAMOTO ,  Shinji SUGIURA ,  Kazumi SHIN ,  Fumiki YANAGAWA ,  Toshiyuki KANAMORI

IPC: C12M3/06 ,  B01J19/00 ,  C09J167/02 ,  G01N35/00

CPC classification number: C12M23/16 ,  B01J19/0046 ,  B01J19/0093 ,  B01L2300/0819 ,  B33Y10/00 ,  C09J4/06 ,  C09J167/02 ,  C12M23/22 ,  C12M33/00 ,  G01N35/00029 ,  G01N2035/00158

Abstract: A method for producing a chip for cell culture that enables production of a microchannel structure, enables mass production at low cost, and also has functions suited to cell culture. The method includes forming a photosensitive adhesive layer by applying a photosensitive adhesive to a first substrate having transparency, thus obtaining a 3D printing substrate, 3D printing steps that employ vat photopolymerization, forming a first adhesive layer by depositing a first adhesive on a second substrate having transparency, thus obtaining a cell culture channel top plate, and bonding the cell culture channel and the cell culture channel top plate together via the first adhesive layer, and then performing thermocompression bonding to obtain a microchannel structural body having a hollow structure, wherein the first adhesive is a polyester-based resin having a Tg value of 5° C. or higher.

公开(公告)号：US09958465B2

公开(公告)日：2018-05-01

申请号：US15403896

申请日：2017-01-11

Applicant: Illumina, Inc. ,  Illumina Singapore Pte Ltd

Inventor： Beng Keong Ang ,  Heng Kuang Cheng ,  John M. Beierle ,  Bradley Kent Drews ,  David Kaplan

IPC: G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  G01N21/64 ,  G01N35/02

CPC classification number: G01N35/00029 ,  B01L3/502715 ,  B01L9/527 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6456 ,  G01N21/6486 ,  G01N35/0099 ,  G01N35/021 ,  G01N35/04 ,  G01N35/1002 ,  G01N35/1095 ,  G01N2021/6482 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/0465

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

公开(公告)号：US20180088023A1

公开(公告)日：2018-03-29

申请号：US15815532

申请日：2017-11-16

Applicant: DeNovo Sciences, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  C12M1/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1484 ,  B01L3/021 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0848 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2400/086 ,  C12M47/04 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N1/405 ,  G01N1/4077 ,  G01N15/1475 ,  G01N35/00029 ,  G01N35/1011 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00158 ,  G01N2035/1039 ,  G06K9/00127 ,  G06K9/2027 ,  G06K9/2036 ,  G06K9/209

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.