公开(公告)号：US10584312B2

公开(公告)日：2020-03-10

申请号：US13257961

申请日：2010-03-03

申请人： Kyung Sun Kang ,  Kyoung Hwan Roh

发明人： Kyung Sun Kang ,  Kyoung Hwan Roh

IPC分类号： C12N5/0789 ,  A61K35/12 ,  A61K35/44 ,  C12N5/073

摘要： The present invention relates to a method for isolating pluripotent/multipotent stem cells derived from umbilical cord blood, characterized by culturing mononuclear cells isolated from umbilical cord blood in a culture vessel containing fibronectin and then harvesting stem cells from the culture, the umbilical cord blood-derived pluripotent/multipotent stem cells isolated thereby; and a cell therapeutic agent containing the pluripotent/multipotent stem cells derived from umbilical cord blood or cells differentiated therefrom. The present invention also relates to a novel culture media for stem cells, a culture method for stem cells which is characterized by culturing and proliferating stem cells in the culture media, and a method for increasing sternness of stem cells which is characterized by a sphere culture or a three-dimensional culture of stem cells.

公开(公告)号：US20120021509A1

公开(公告)日：2012-01-26

申请号：US13257961

申请日：2010-03-03

申请人： Kyung Sun Kang ,  Kyoung Hwan Roh

发明人： Kyung Sun Kang ,  Kyoung Hwan Roh

IPC分类号： C12N5/0789

摘要： The present invention relates to a method for isolating pluripotent/multipotent stem cells derived from umbilical cord blood, characterized by culturing monocytes isolated from umbilical cord blood in a culture vessel containing fibronectin and then harvesting stem cells from the culture, the umbilical cord blood-derived pluripotent/multipotent stem cells isolated thereby; and a cell therapeutic agent containing the pluripotent/multipotent stem cells derived from umbilical cord blood or cells differentiated therefrom. The present invention also relates to a novel culture media for stem cells, a culture method for stem cells which is characterized by culturing and proliferating stem cells in the culture media, and a method for increasing stemness of stem cells which is characterized by a sphere culture or a three-dimensional culture of stem cells.

摘要翻译： 本发明涉及一种用于分离源自脐带血的多能/多能干细胞的方法，其特征在于在含有纤粘连蛋白的培养容器中培养从脐带血分离的单核细胞，然后从培养物中收获干细胞，脐带血衍生 由此分离的多能/多能干细胞; 以及含有衍生自脐带血的多能/多能干细胞或与其分化的细胞的细胞治疗剂。 本发明还涉及用于干细胞的新型培养基，干细胞的培养方法，其特征在于培养培养基中培养和增殖干细胞，以及提高干细胞干细胞的方法，其特征在于球培养 或干细胞的三维培养。