公开(公告)号：US3639558A

公开(公告)日：1972-02-01

申请号：US3639558D

申请日：1968-02-19

申请人： LOUIS CSIZMAS ,  VIRENDRA PATEL

发明人： CSIZMAS LOUIS ,  PATEL VIRENDRA

IPC分类号： G01N33/543 ,  G01N33/554 ,  G01N31/00 ,  G01N33/16

CPC分类号： G01N33/554 ,  G01N33/54313 ,  Y10S435/832 ,  Y10S435/839 ,  Y10S435/849 ,  Y10S435/853 ,  Y10S435/874 ,  Y10S436/811 ,  Y10S436/814 ,  Y10S436/818 ,  Y10S436/826

摘要： REACTIVE PARTICLES OF CONTROLLED REACTIVITY AND SIZE WHICH ARE CAPABLE OF DIRECT CHEMICAL ATTACHMENT TO BIOLOGICAL SUBSTANCES CAN BE MADE BY COATING SOLID CARRIER PARTICLES WITH AT LEAST ONE COAT OF A PROTEINACEOUS MATERIAL BY THE USE OF A FIRST COUPLING AGENT AND THEN ATTACHING TO SAID PROTEINACEOUS COAT THROUGH COVALENT BONDING A SECOND COUPLING AGENT CAPABLE OF ATTACHMENT TO BIOLOGICAL SUBSTANCES THROUGH COVALENT BONDING. INSOLUBILZED BIOLOGICAL REAGENTS MAY BE PRODUCED FROM SUCH REACTIVE PARTICLES MERELY BY MIXING THESE PARTICLES WITH BIOLOGICAL SUBSTANCES WHICH WILL BE BOUND THERETO THROUGH THE SECOND COUPLING AGENT. WITH THE USE OF DIFFERENT COUPLING AGENTS VARYING REACTIVITY CAN BE ATTAINED, AND THE DESIRED FINAL PARTICLE SIZE WILL DEPEND UPON THE NUMBER OF COATS OF THE PROTEINACEOUS MATERIAL EMPLOYED.