公开(公告)号：US08802381B2

公开(公告)日：2014-08-12

申请号：US13846172

申请日：2013-03-18

申请人： John O'Connor ,  Steven Birken ,  Galina Kovalevskaya

发明人： John O'Connor ,  Steven Birken ,  Galina Kovalevskaya

IPC分类号： G01N31/00 ,  G01N33/53

CPC分类号： G01N33/76 ,  C07K16/26 ,  G01N33/57434 ,  G01N33/57449 ,  G01N2333/59 ,  G01N2800/368 ,  Y10S436/814 ,  Y10S436/818

摘要： The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

摘要翻译： 本发明提供了通过确定样品中hCG的早期妊娠相关分子同种型的量来预测受试者的妊娠结局的方法。 本发明还提供了一种用于确定样品中人绒毛膜促性腺激素（hCG）的早期妊娠相关分子同种型的量的方法。 本发明还提供了用于确定样品中早期妊娠相关hCG的量的诊断试剂盒。 本发明另外提供了与人绒毛膜促性腺激素的早期怀孕相关分子同种型特异性结合的抗体。 最后，本发明提供了检测样品中滋养层细胞或非滋养层恶性肿瘤的方法。

公开(公告)号：US07993858B2

公开(公告)日：2011-08-09

申请号：US12807513

申请日：2010-09-07

申请人： John O'Connor ,  Steven Birken ,  Galina Kovalevskaya

发明人： John O'Connor ,  Steven Birken ,  Galina Kovalevskaya

IPC分类号： G01N1/00 ,  G01N33/53

CPC分类号： G01N33/76 ,  C07K16/26 ,  G01N33/57434 ,  G01N33/57449 ,  G01N2333/59 ,  G01N2800/368 ,  Y10S436/814 ,  Y10S436/818

摘要： The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

摘要翻译： 本发明提供了通过确定样品中hCG的早期妊娠相关分子同种型的量来预测受试者的妊娠结局的方法。 本发明还提供了一种用于确定样品中人绒毛膜促性腺激素（hCG）的早期妊娠相关分子同种型的量的方法。 本发明还提供了用于确定样品中早期妊娠相关hCG的量的诊断试剂盒。 本发明另外提供了与人绒毛膜促性腺激素的早期怀孕相关分子同种型特异性结合的抗体。 最后，本发明提供了检测样品中滋养层细胞或非滋养层恶性肿瘤的方法。

公开(公告)号：US20100099117A1

公开(公告)日：2010-04-22

申请号：US12649156

申请日：2009-12-29

申请人： Eeva-Marja RUTANEN

发明人： Eeva-Marja RUTANEN

IPC分类号： G01N33/53

CPC分类号： G01N33/689 ,  G01N2800/368 ,  Y10S435/975 ,  Y10S436/814

摘要： The invention relates to a diagnostic method for detecting susceptibility to delivery, and to a test kit for this purpose. A low, but higher than baseline level concentration of Insulin-like Growth Factor Binding Protein 1 (IGFBP-1), which is due to leakage from decidual cells, is detected by an immunological assay in a vaginal secretion sample.

摘要翻译： 本发明涉及一种用于检测易感性的诊断方法以及用于该目的的检测试剂盒。 通过阴道分泌物样品中的免疫学检测来检测由于蜕膜细胞渗漏引起的胰岛素样生长因子结合蛋白1（IGFBP-1）的低浓度但高于基线水平的浓度。

公开(公告)号：US07629178B2

公开(公告)日：2009-12-08

申请号：US11923930

申请日：2007-10-25

申请人： Paul James Davis ,  Michael Evans Prior ,  Keith May

发明人： Paul James Davis ,  Michael Evans Prior ,  Keith May

IPC分类号： G01N33/558 ,  C12Q1/00 ,  G01N33/53 ,  C12M1/34 ,  C12M3/00 ,  G01N21/00 ,  G01N15/06 ,  C12Q1/68 ,  B32B5/02

CPC分类号： G01N33/54386 ,  G01N33/558 ,  G01N33/76 ,  Y10S435/805 ,  Y10S435/806 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/81 ,  Y10S436/814 ,  Y10S436/818

摘要： An analytical test device incorporating a dry porous carrier to which a liquid sample, eg. urine, suspected of containing an analyte such as HCG or LH can be applied indirectly, the device also incorporating a labelled specific binding reagent which is freely mobile in the porous carrier when in the moist state, and an unlabelled specific binding reagent which is permanently immobilized in a detection zone on the carrier material, the labelled and unlabelled specific binding reagents being capable of participating in either a sandwich reaction or a competition reacts on in the presence of the analyte, in which prior to the application to the device of a liquid sample suspected of containing the analyte, the labelled specific binding reagent is retained in the dry state in a macroporous body, eg. of plastics material having a pore size of 10 microns or greater, through which the applied liquid sample must pass en route to the porous carrier material, the labelled specific binding reagent being freely soluble or dispersible in any liquid sample which enters the macroporous body.

摘要翻译： 包含干多孔载体的分析测试装置，液体样品例如 怀疑含有分析物如HCG或LH的尿液可以间接施用，该装置还包含标记的特异性结合试剂，其在处于潮湿状态时可在多孔载体中自由移动，以及永久固定的未标记的特异性结合试剂 在载体材料上的检测区域中，能够参与夹心反应或竞争性的标记和未标记的特异性结合试剂在分析物存在下反应，其中在施加到液体样品的装置之前 怀疑含有分析物，标记的特异性结合试剂在大孔体内保持干燥状态，例如。 的孔径为10微米或更大的塑料材料，所施加的液体样品通过该材料必须通过多孔载体材料，标记的特异性结合试剂可自由溶解或分散在进入大孔体的任何液体样品中。

公开(公告)号：US07575861B2

公开(公告)日：2009-08-18

申请号：US10496164

申请日：2002-11-20

申请人： Matthew C. Lucy ,  Nagappan Mathialagan

发明人： Matthew C. Lucy ,  Nagappan Mathialagan

IPC分类号： G01N33/53

CPC分类号： G01N33/689 ,  G01N33/743 ,  G01N2800/368 ,  Y10S436/814 ,  Y10S436/817

摘要： The invention provides improved assays for detection of pregnancy. In the assays, pregnancy associated glycoproteins are analyzed in conjunction with progesterone analysis. The techniques of the invention overcome limitations in the prior art by reducing the rate of false positive results. The assays provided by the invention can be implemented to increase the efficiency of commercial animal breeding programs.

摘要翻译： 本发明提供了用于检测妊娠的改进的测定法。 在测定中，与孕激素分析结合分析妊娠相关糖蛋白。 本发明的技术通过降低假阳性结果的比例来克服现有技术中的局限性。 可以实施本发明提供的测定以提高商业动物育种程序的效率。

公开(公告)号：US20080274564A1

公开(公告)日：2008-11-06

申请号：US12174348

申请日：2008-07-16

申请人： Vito J. D'Aurora

发明人： Vito J. D'Aurora

IPC分类号： G01N33/76

CPC分类号： A61D17/006 ,  G01N33/74 ,  G01N33/743 ,  Y10S436/811 ,  Y10S436/814

摘要： A method of testing an animal for pregnancy or identifying the sex of the animal comprising the steps of first, providing a first vessel containing a liquid and having a removable surface wherein said removable surface is at least partially coated with an antibody and then introducing a bodily fluid from the female animal into said first vessel so that said bodily fluid contacts the liquid and then manipulating the first vessel so that the liquid contacts the antibody. Then, a second vessel containing a reporter hormone solution is provided and the removable surface from the first vessel is displaced to the second vessel and manipulating the second vessel so that the reporter hormone solution contacts the removable surface. Then, a third vessel containing an indicating solution which has an appearance which is related to the amount of the reporter hormone contacted is provided, and the removable surface is displaced from the second vessel to the third vessel. The third vessel is manipulated so that the indicating solution contacts the removable surface. Then, a determination is made regarding the pregnancy or sex based on the appearance of the indicating solution.

摘要翻译： 一种测试动物用于妊娠或鉴定动物性别的方法，包括以下步骤：首先提供含有液体的第一容器并具有可移除表面，其中所述可移除表面至少部分地涂覆有抗体，然后引入身体 流体从雌性动物进入所述第一容器，使得所述体液接触液体，然后操纵第一容器，使得液体接触抗体。 然后，提供含有报告激素溶液的第二容器，将来自第一容器的可移除表面置换到第二容器并操纵第二容器，使得报道激素溶液接触可除去的表面。 然后，提供含有与接触的报告激素的量相关的外观的指示液的第三容器，并且可移除表面从第二容器移位到第三容器。 操作第三容器使得指示溶液接触可除去的表面。 然后，基于指示溶液的外观判定怀孕或性别。

公开(公告)号：US07384796B2

公开(公告)日：2008-06-10

申请号：US10328403

申请日：2002-12-23

申请人： Paul James Davis ,  Michael Evans Prior ,  Keith May

发明人： Paul James Davis ,  Michael Evans Prior ,  Keith May

IPC分类号： G01N33/558 ,  G01N33/53 ,  G01N21/00 ,  G01N15/06 ,  C12Q1/00 ,  C12Q1/68 ,  C12M1/34 ,  C12M3/00 ,  B32B5/02

CPC分类号： G01N33/54386 ,  G01N33/558 ,  G01N33/76 ,  Y10S435/805 ,  Y10S435/806 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/81 ,  Y10S436/814 ,  Y10S436/818

摘要： An analytical test device incorporating a dry porous carrier to which a liquid sample, eg. urine, suspected of containing an analyte such as HCG or LH can be applied indirectly, the device also incorporating a labelled specific binding reagent which is freely mobile in the porous carrier when in the moist state, and an unlabelled specific binding reagent which is permanently immobilised in a detection zone on the carrier material, the labelled and unlabelled specific binding reagents being capable of participating in either a sandwich reaction or a competition reaction in the presence of the analyte, in which prior to the application to the device of a liquid sample suspected of containing the analyte, the labelled specific binding reagent is retained in the dry state in a macroporous body, eg. of plastics material having a pore size of 10 microns or greater, through which the applied liquid sample must pass en route to the porous carrier material, the labelled specific binding reagent being freely soluble or dispersible in any liquid sample which enters the macroporous body.

摘要翻译： 包含干多孔载体的分析测试装置，液体样品例如 怀疑含有分析物如HCG或LH的尿液可以间接施用，该装置还包含标记的特异性结合试剂，其在处于潮湿状态时可在多孔载体中自由移动，以及永久固定的未标记的特异性结合试剂 在载体材料上的检测区域中，标记和未标记的特异性结合试剂能够在分析物存在下参与夹心反应或竞争反应，其中在将液体样品的装置应用于怀疑 含有分析物时，标记的特异性结合试剂在大孔体内保持干燥状态，例如。 的孔径为10微米或更大的塑料材料，所施加的液体样品通过该材料必须通过多孔载体材料，标记的特异性结合试剂可自由溶解或分散在进入大孔体的任何液体样品中。

公开(公告)号：US06962808B2

公开(公告)日：2005-11-08

申请号：US09910628

申请日：2001-07-20

申请人： Judith L. Erb ,  James G. Downward, IV ,  John R. Erb-Downward ,  James L. Witliff

发明人： Judith L. Erb ,  James G. Downward, IV ,  John R. Erb-Downward ,  James L. Witliff

IPC分类号： G01N21/77 ,  C12M1/34

CPC分类号： G01N21/648 ,  G01N21/7703 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/814 ,  Y10S436/815 ,  Y10S436/817

摘要： There is provided an apparatus for screening pharmacological agents for agents which induce regression of cancer. The apparatus includes an evanescent sensing device, at least one sensor having affixed to its surface molecules of a first type, which have affinity for molecules of a biological receptor, the surface molecule and receptor molecule combination having the effect that, in vivo, the binding affects the rate of transcription of gene products, and a molecular tag wherein the molecular tag is bound to the sensor wherein the binding between molecules of the first type and molecules the biological receptor cause the tag to produce a alteration in signal recorded by the evanescent sensing device, the tag also being bound to molecules of a second type, the molecules of the second type having affinity for the receptor molecules. Also provided is a method for screening pharmacological agents to determine agents which induce regression of cancer by contacting extract from a tumor tissue biopsy with a molecular tag, thereby causing the tag to bind to receptor molecules present in the tumor tissue biopsy, flowing the tag sample extract through a sensor, as set forth above, and recording the time course of signal observed by the evanescent sensing device, introducing pharmacological agents to be accessed to the tag sample extract, flowing the tag sample extract through the sensor again and recoding the time course of signal observed by the evanescent sensing device, and using the data to evaluate the impact of the pharmacological agents on the rate of transcription of gene products.

摘要翻译： 提供了用于筛选诱导癌症消退的药剂的药理学试剂的装置。 所述装置包括消逝传感装置，至少一个传感器固定在其表面上具有第一类型的分子，其具有对生物受体分子的亲和力，所述表面分子和受体分子组合具有在体内结合 影响基因产物的转录速率和分子标签，其中分子标签与传感器结合，其中第一类型的分子与分子之间的结合生物受体导致标签产生由ev逝感测记录的信号的变化 该标签也与第二类型的分子结合，第二类型的分子对受体分子具有亲和力。 还提供了一种用于筛选药理学试剂以确定通过将来自肿瘤组织活检的提取物与分子标签接触来诱导癌症消退的试剂的方法，从而使标签与存在于肿瘤组织活组织检查中的受体分子结合，使标签样品 通过如上所述的传感器提取并记录由ev逝感测装置观察到的信号的时间过程，将待接入的药剂引入标签样品提取物，再次使标签样品提取物再次通过传感器并重新编码时间过程 的消逝感测装置观察到的信号，并使用数据来评估药理学试剂对基因产物转录速率的影响。

公开(公告)号：US20050042702A1

公开(公告)日：2005-02-24

申请号：US10931956

申请日：2004-09-01

申请人： John Connor ,  Steven Birken ,  Galina Kovalevskaya

发明人： John Connor ,  Steven Birken ,  Galina Kovalevskaya

IPC分类号： G01N33/53 ,  C07K16/26 ,  C12N15/02 ,  G01N33/577 ,  G01N33/76

CPC分类号： G01N33/76 ,  C07K16/26 ,  G01N33/57434 ,  G01N33/57449 ,  G01N2333/59 ,  G01N2800/368 ,  Y10S436/814 ,  Y10S436/818

摘要： The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample.

摘要翻译： 本发明提供了通过确定样品中hCG的早期妊娠相关分子同种型的量来预测受试者的妊娠结局的方法。 本发明还提供了一种用于确定样品中人绒毛膜促性腺激素（hCG）的早期妊娠相关分子同种型的量的方法。 本发明还提供了用于确定样品中早期妊娠相关hCG的量的诊断试剂盒。 本发明另外提供了与人绒毛膜促性腺激素的早期怀孕相关分子同种型特异性结合的抗体。 最后，本发明提供了检测样品中滋养层细胞或非滋养层恶性肿瘤的方法。

公开(公告)号：US20040241752A1

公开(公告)日：2004-12-02

申请号：US10877122

申请日：2004-06-25

发明人： Emory V. Anderson ,  Jerome Lapointe ,  Ricardo Martinez ,  Gail Marzolf ,  Ronald Pong ,  Lynn Jones ,  Robert O. Hussa ,  Edward Nemec ,  Andrew E. Senyei ,  Duane DeSieno

IPC分类号： G01N033/53 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC分类号： G01N21/474 ,  G01N21/8483 ,  G01N33/558 ,  G01N33/6887 ,  G01N33/689 ,  G01N2333/78 ,  G01N2800/368 ,  G06F19/00 ,  G06F19/321 ,  G16H10/40 ,  G16H10/60 ,  G16H15/00 ,  G16H50/20 ,  Y02A90/26 ,  Y10S436/811 ,  Y10S436/814

摘要： Systems and methods for medical diagnosis or risk assessment for a patient are provided. These systems and methods are designed to be employed at the point of care, such as in emergency rooms and operating rooms, or in any situation in which a rapid and accurate result is desired. The systems and methods process patient data, particularly data from point of care diagnostic tests or assays, including immunoassays, electrocardiograms, X-rays and other such tests, and provide an indication of a medical condition or risk or absence thereof. The systems include an instrument for reading or evaluating the test data and software for converting the data into diagnostic or risk assessment information.

摘要翻译： 提供了用于医疗诊断或患者风险评估的系统和方法。 这些系统和方法被设计为在护理点使用，例如在急诊室和手术室中，或在需要快速和准确的结果的任何情况下使用。 系统和方法处理患者数据，特别是来自护理诊断测试或测定的数据，包括免疫测定，心电图，X射线和其他此类测试，并提供医疗状况或其风险或不存在的指示。 系统包括用于读取或评估测试数据的工具，以及用于将数据转换为诊断或风险评估信息的软件。