公开(公告)号：US20050282197A1

公开(公告)日：2005-12-22

申请号：US11121290

申请日：2005-05-03

申请人： Lindsey Foreman ,  Ian Evett ,  Susan Pope ,  John Buckleton ,  James Curran ,  Christopher Triggs

发明人： Lindsey Foreman ,  Ian Evett ,  Susan Pope ,  John Buckleton ,  James Curran ,  Christopher Triggs

IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： G16B20/00 ,  G01N2550/00

摘要： Methods for establishing the genotype of a DNA sample, and methods for investigating the potential sources of a DNA sample arising from a plurality of source, are provided, the methods being based on a method including: analysing the sample to produce a data profile for the sample for a locus; proposing a suggested genotype; generating a first stage profile for the locus for the suggested genotype; adjusting the first stage profile to account for one or more factors to give a simulation profile; and comparing the data profile and the simulation profile to provide an indication of the likelihood of the data profile given the suggested genotype. The methods in effect make adjustments to take the first stage profile, potentially through one or more intervening profiles, to the simulation profile, the simulation profile being an anticipation of the data profile which would be expected to occur for that suggested genotype in practice. The methods potentially include adjustments for one or more of preferential amplification and/or stutter and/or allele drop out and/or allele drop in and/or stochastic components and/or noise and/or preferential degradation and/or the relative contributions from the sources.

摘要翻译： 提供了建立DNA样品的基因型的方法以及用于研究由多个源产生的DNA样品的潜在来源的方法，所述方法基于一种方法，包括：分析样品以产生用于 一个地点的样本 提出建议的基因型; 为所建议的基因型产生基因座的第一阶段谱; 调整第一阶段轮廓以考虑一个或多个因素以给出模拟轮廓; 并且比较数据简档和模拟简档，以提供给定建议的基因型的数据简档的可能性的指示。 这些方法实际上可以通过一个或多个介入简档将模拟简档作为第一阶段简档进行调整，该模拟简档是预期将在实践中对该建议的基因型预期发生的数据简档。 这些方法可能包括对优先扩增和/或口吃和/或等位基因脱落和/或等位基因落入和/或随机组分中和/或噪声和/或优先降解和/或来自 来源。

公开(公告)号：US20090326830A1

公开(公告)日：2009-12-31

申请号：US12431221

申请日：2009-04-28

申请人： Lindsey Foreman ,  Ian Evett ,  Susan Pope ,  John Buckleton ,  James Curran ,  Christopher Triggs

发明人： Lindsey Foreman ,  Ian Evett ,  Susan Pope ,  John Buckleton ,  James Curran ,  Christopher Triggs

IPC分类号： G06F19/00

CPC分类号： G16B20/00 ,  G01N2550/00

摘要： Methods for establishing the genotype of a DNA sample, and methods for investigating the potential sources of a DNA sample arising from a plurality of source, are provided, the methods being based on a method including: analysing the sample to produce a data profile for the sample for a locus; proposing a suggested genotype; generating a first stage profile for the locus for the suggested genotype; adjusting the first stage profile to account for one or more factors to give a simulation profile; and comparing the data profile and the simulation profile to provide an indication of the likelihood of the data profile given the suggested genotype. The methods in effect make adjustments to take the first stage profile, potentially through one or more intervening profiles, to the simulation profile, the simulation profile being an anticipation of the data profile which would be expected to occur for that suggested genotype in practice. The methods potentially include adjustments for one or more of preferential amplification and/or stutter and/or allele drop out and/or allele drop in and/or stochastic components and/or noise and/or preferential degradation and/or the relative contributions from the sources.

摘要翻译： 提供了建立DNA样品的基因型的方法以及用于研究由多个源产生的DNA样品的潜在来源的方法，所述方法基于一种方法，包括：分析样品以产生用于 一个地点的样本 提出建议的基因型; 为所建议的基因型产生基因座的第一阶段谱; 调整第一阶段轮廓以考虑一个或多个因素以给出模拟轮廓; 并且比较数据简档和模拟简档，以提供给定建议的基因型的数据简档的可能性的指示。 这些方法实际上可以通过一个或多个介入简档将模拟简档作为第一阶段简档进行调整，该模拟简档是预期将在实践中对该建议的基因型预期发生的数据简档。 这些方法可能包括对优先扩增和/或口吃和/或等位基因脱落和/或等位基因落入和/或随机组分中和/或噪声和/或优先降解和/或来自 来源。