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公开(公告)号：US08206930B2
公开(公告)日：2012-06-26
申请号：US13199093
申请日：2011-08-18
申请人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
发明人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.
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公开(公告)号：US08026056B2
公开(公告)日：2011-09-27
申请号：US12156029
申请日：2008-05-29
申请人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
发明人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.
摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。
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公开(公告)号：US20090275023A1
公开(公告)日：2009-11-05
申请号：US12156029
申请日：2008-05-29
申请人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
发明人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.
摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。
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公开(公告)号：US20120122092A1
公开(公告)日：2012-05-17
申请号：US13199093
申请日：2011-08-18
申请人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
发明人： Melanie Feola , Martin Adelson , Eli Mordechai , Lisa Novak
IPC分类号： C12Q1/68
摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.
摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中使用的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。
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