公开(公告)号：US09173383B2

公开(公告)日：2015-11-03

申请号：US12918676

申请日：2009-02-20

申请人： Lishan Su ,  Liguo Zhang ,  Michael Washburn

发明人： Lishan Su ,  Liguo Zhang ,  Michael Washburn

IPC分类号： A01K67/027 ,  C12N9/64 ,  C12N15/85

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/0337 ,  C12N9/6475 ,  C12N15/02 ,  C12N15/8509 ,  G01N33/5088

摘要： Provided herein is a recombinant non-human mammal having an immune system including human immune cells and having a liver including human liver cells, and methods for producing the same. Also provided are methods of screening a compound for activity in treating hepatitis, comprising: administering a test compound to a recombinant non-human mammal as described herein; and then detecting the presence or absence of said activity in said mammal (e.g., by biochemical assay), said presence of said activity in said mammal indicating that said compound has activity in treating hepatitis. Methods of making fusion cells useful for the production of human monoclonal antibodies are also provided.

摘要翻译： 本文提供了具有免疫系统的重组非人哺乳动物，其包括人免疫细胞并具有包括人肝细胞的肝脏及其制备方法。 还提供了筛选化合物用于治疗肝炎的活性的方法，其包括：向本文所述的重组非人哺乳动物施用测试化合物; 然后检测所述哺乳动物中所述活性的存在或不存在（例如，通过生化测定），所述哺乳动物中所述活性的存在表明所述化合物具有治疗肝炎的活性。 还提供了制备用于生产人单克隆抗体的融合细胞的方法。

公开(公告)号：US20110041192A1

公开(公告)日：2011-02-17

申请号：US12918676

申请日：2009-02-20

申请人： Lishan Su ,  Liguo Zhang ,  Michael Washburn

发明人： Lishan Su ,  Liguo Zhang ,  Michael Washburn

IPC分类号： A01K67/00 ,  G01N33/53 ,  C12N5/16 ,  G01N33/00 ,  C12N15/63

CPC分类号： A01K67/0271 ,  A01K67/0275 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/0337 ,  C12N9/6475 ,  C12N15/02 ,  C12N15/8509 ,  G01N33/5088

摘要： Provided herein is a recombinant non-human mammal having an immune system including human immune cells and having a liver including human liver cells, and methods for producing the same. Also provided are methods of screening a compound for activity in treating hepatitis, comprising: administering a test compound to a recombinant non-human mammal as described herein; and then detecting the presence or absence of said activity in said mammal (e.g., by biochemical assay), said presence of said activity in said mammal indicating that said compound has activity in treating hepatitis. Methods of making fusion cells useful for the production of human monoclonal antibodies are also provided.

摘要翻译： 本文提供了具有免疫系统的重组非人哺乳动物，其包括人免疫细胞并具有包括人肝细胞的肝脏及其制备方法。 还提供了筛选化合物用于治疗肝炎的活性的方法，其包括：向本文所述的重组非人哺乳动物施用测试化合物; 然后检测所述哺乳动物中所述活性的存在或不存在（例如，通过生化测定），所述哺乳动物中所述活性的存在表明所述化合物具有治疗肝炎的活性。 还提供了制备用于生产人单克隆抗体的融合细胞的方法。

公开(公告)号：US5612018A

公开(公告)日：1997-03-18

申请号：US183178

申请日：1994-01-18

申请人： Mark L. Bonyhadi ,  Hideto Kaneshima ,  Joseph M. McCune ,  Reiko Namikawa ,  Lishan Su

发明人： Mark L. Bonyhadi ,  Hideto Kaneshima ,  Joseph M. McCune ,  Reiko Namikawa ,  Lishan Su

IPC分类号： A01K67/027 ,  A61K49/00 ,  C12N15/85

CPC分类号： A61K49/0004 ,  A01K67/0271 ,  C12N2517/00

摘要： A method is provided for screening compounds for the ability to supress thymocyte depletion in thymuses of HIV-infected individuals, particularly enhancing the CD4.sup.+ -expressing population as compared to an untreated individual. Particularly, drugs are provided which allow for this result, cyclosporine A being exemplary.

摘要翻译： 提供了一种筛选化合物以抑制HIV感染个体的胸腺中胸腺细胞消耗的能力的方法，特别是与未处理的个体相比增强了CD4 +表达的群体。 特别地，提供了允许该结果的药物，环孢菌素A是示例性的。