公开(公告)号：US06221821B1

公开(公告)日：2001-04-24

申请号：US09271778

申请日：1999-03-18

申请人： Allan Svendsen ,  Louise Jørgensen

发明人： Allan Svendsen ,  Louise Jørgensen

IPC分类号： C12N908

CPC分类号： C12N9/0065 ,  C09D5/14 ,  C11D3/38654

摘要： Variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A, L, I, Q, M, E, D; A399G; F397N, Y, E, Q; P395A, S; R360A, L, I, Q, M, E, D; K353Q, M; S402A, T, V, S; D292L; A501S; W350F, Y; V495A, T, V, S; K394 A, L, I, Q, M, E, D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.

摘要翻译： 母体含钒卤代过氧化物酶的变体，其变体具有卤代过氧化物酶活性和改变的pH最佳值，并且在与以下至少一个位置相对应的位置包含突变：R490A，L，I，Q，M，E，D; A399G; F397N，Y，E，Q; P395A，S; R360A，L，I，Q，M，E，D; K353Q，M; S402A，T，V，S; D292L; A501S; W350F，Y; V495A，T，V，S; K394 A，L，I，Q，M，E，D; 其中母体卤代过氧化物酶具有SEQ ID No.1所示的氨基酸序列，或者母体过氧化氢酶具有与SEQ ID No.1至少80％同源的氨基酸序列。

公开(公告)号：US06372465B1

公开(公告)日：2002-04-16

申请号：US09788871

申请日：2001-02-20

申请人： Allan Svendsen ,  Louise Jørgensen

发明人： Allan Svendsen ,  Louise Jørgensen

IPC分类号： C12N908

摘要： The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.