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19 条记录 (耗时 0.019 秒)

    Catalases
    5.
    发明授权
    Catalases 有权
    过氧化氢酶

    公开(公告)号：US06410290B1

    公开(公告)日：2002-06-25

    申请号：US09412347

    申请日：1999-10-05

    申请人： Dan E. Robertson ,  Indrajit Sanyal ,  Robert S. Adhikary

    发明人： Dan E. Robertson ,  Indrajit Sanyal ,  Robert S. Adhikary

    IPC分类号： C12N908

    CPC分类号： C12N9/0065 ,  A01K2217/05 ,  C12Q1/30

    摘要： Catalase enzymes derived from bacteria from the genera Alcaligenes (Deleya) and Microscilla are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized to destroy or detect hydrogen peroxide, e.g., in production of glyoxylic acid and in glucose sensors, and in processes where hydrogen peroxide is used as a bleaching or antibacterial agent, e.g. in contact lens cleaning, in bleaching steps in pulp and paper preparation and in the pasteurization of dairy products.

    摘要翻译： 公开了源自产碱杆菌属（Deleya）和Microscilla的细菌的过氧化氢酶。 酶由天然或重组宿主细胞产生，可​​用于破坏或检测过氧化氢，例如在生产乙醛酸和葡萄糖传感器时，以及过氧化氢用作漂白剂或抗菌剂的过程。 在隐形眼镜清洁中，在纸浆和纸制备中的漂白步骤和乳制品的巴氏消毒中。

    Haloperoxidases with altered pH profiles
    6.
    发明授权
    Haloperoxidases with altered pH profiles 有权

    公开(公告)号：US06372465B1

    公开(公告)日：2002-04-16

    申请号：US09788871

    申请日：2001-02-20

    申请人： Allan Svendsen ,  Louise Jørgensen

    发明人： Allan Svendsen ,  Louise Jørgensen

    IPC分类号： C12N908

    摘要： The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.

    Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof
    7.
    发明授权
    Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof 有权
    与新型转酮醇酶有关的核酸和氨基酸序列及其表达方法

    公开(公告)号：US06190895B1

    公开(公告)日：2001-02-20

    申请号：US09146221

    申请日：1998-09-01

    申请人： Rodney Bruce Croteau ,  Mark Raymond Wildung ,  Bernd Markus Lange ,  David G. McCaskill

    发明人： Rodney Bruce Croteau ,  Mark Raymond Wildung ,  Bernd Markus Lange ,  David G. McCaskill

    IPC分类号： C12N908

    CPC分类号： C12N9/1022 ,  C12N15/8243

    摘要： cDNAs encoding 1-deoxyxylulose-5-phosphate synthase from peppermint (Mentha piperita) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7) are provided which code for the expression of 1-deoxyxylulose-5-phosphate synthase from plants. In another aspect the present invention provides for isolated, recombinant DXPS proteins, such as the proteins having the sequences set forth in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8. In other aspects, replicable recombinant cloning vehicles are provided which code for plant 1-deoxyxylulose-5-phosphate synthases, or for a base sequence sufficiently complementary to at least a portion of 1-deoxyxylulose-5-phosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a plant 1-deoxyxylulose-5-phosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant 1-deoxyxylulose-5-phosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant 1-deoxyxylulose-5-phosphate synthase may be used to obtain expression or enhanced expression of 1-deoxyxylulose-5-phosphate synthase in plants in order to enhance the production of 1-deoxyxylulose-5-phosphate, or its derivatives such as isopentenyl diphosphate (BP), or may be otherwise employed for the regulation or expression of 1-deoxyxylulose-5-phosphate synthase, or the production of its products.

    摘要翻译： 已经分离并测序了从薄荷（薄荷胡椒粉）中编码1-脱氧纤维素-5-磷酸合成酶的cDNA，并测定了相应的氨基酸序列。 因此，提供编码从植物中表达1-脱氧纤维素-5-磷酸合酶的分离的DNA序列（SEQ ID NO：3，SEQ ID NO：5，SEQ ID NO：7）。 在另一方面，本发明提供了分离的重组DXPS蛋白，例如具有SEQ ID NO：4，SEQ ID NO：6和SEQ ID NO：8所示序列的蛋白质。 在其他方面，提供可编码的重组克隆载体，其编码植物1-脱氧基纤维素-5-磷酸合酶，或编码与至少一部分1-脱氧基纤维素-5-磷酸合酶DNA或RNA足够互补的碱基序列，以使得能够进行杂交 随之而来。 在另一方面，提供已经用编码植物1-脱氧基纤维素-5-磷酸合酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组1-脱氧纤维素-5-磷酸合酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 重组1-脱氧核糖-5-磷酸合成酶可用于获得植物中1-脱氧纤维素-5-磷酸合成酶的表达或增强，以增强1-脱氧纤维素-5-磷酸或其衍生物如异戊烯基 二磷酸酯（BP），或者可以另外用于1-脱氧氧基-5-磷酸合酶的调节或表达，或其产物的生产。