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    Method of isolation of lysozyme
    3.
    发明授权
    Method of isolation of lysozyme 失效
    溶菌酶的分离方法

    公开(公告)号：US3940317A

    公开(公告)日：1976-02-24

    申请号：US348780

    申请日：1973-04-06

    申请人： Friedrich D. Katz ,  Louis Fishman ,  Milton Levy

    发明人： Friedrich D. Katz ,  Louis Fishman ,  Milton Levy

    IPC分类号： C12N9/36 ,  C07G7/02 ,  C07G7/022 ,  C07G7/026

    CPC分类号： C12N9/2462 ,  Y10S435/815

    摘要： Lysozyme from human, avian, non-human mammalian, and vegatable sources is isolated from said sources and provided in a high degree of purity by adsorbing the lysozyme upon chitin obtained from Loligo vulgaris by contacting said chitin with an acidic aqueous suspension of the crude lysozyme containing material, and eluting the pure lysozyme from said chitin with aqueous acid.

    摘要翻译： 通过将所述几丁质与粗溶菌酶的酸性水性悬浮液接触，将来自所述来源的溶菌酶从所述来源分离并通过将通过将溶菌酶吸附到从寻常L itin获得的几丁质上而提供的高度纯度 并用酸水溶液从所述几丁质中洗脱纯溶菌酶。

    Method of separating and purifying two active forms of urokinase using affinity chromatography
    8.
    发明授权
    Method of separating and purifying two active forms of urokinase using affinity chromatography 失效
    使用亲和色谱法分离和纯化尿激酶的两种活性形式的方法

    公开(公告)号：US4066506A

    公开(公告)日：1978-01-03

    申请号：US730728

    申请日：1976-10-08

    申请人： Alan J. Johnson ,  Mercedes E. Soberano ,  Eng Bee Ong ,  Milton Levy

    发明人： Alan J. Johnson ,  Mercedes E. Soberano ,  Eng Bee Ong ,  Milton Levy

    IPC分类号： C12N9/72 ,  C07G7/02 ,  C12D13/10

    CPC分类号： C12N9/6462 ,  C12Y304/21073 ,  Y10S435/814

    摘要： An affinity chromatography method for the separation and pruification of two active forms of urokinase from a crude urokinase preparation. The method employs an extracting agent of agmatine covalently coupled to the surface of a water-insoluble solid support material such as agarose, and three separate specific buffer solutions. A low ionic strength buffer, such as 0.01 molar sodium phosphate buffer, pH 6.0-9.0, is used in preparing the loading solution and for washing the extracting agent. A slightly higher ionic strength buffer, such as 0.02 molar sodium phosphate buffer, pH 6.0-9.0, is used as a first eluant for eluting from the extracting agent a first active form of urokinase which is characterized by a molecular weight of approximately 33,400 and a specific activity of approximately 226,000 CTA units/mg protein. A still higher ionic strength buffer with added salt, such as 0.1 molar sodium phosphate -- 0.4 molar sodium chloride buffer, pH 5.0-8.0, is used as a second eluant for eluting from the extracting agent a second active form of urokinase which is characterized by a molecular weight of approximately 47,000 and a specific activity of approximately 104,000 CTA units/mg protein.