Mass Spectrometry-Based Detection Of Mycotoxins In Meat

    公开(公告)号:US20220268772A1

    公开(公告)日:2022-08-25

    申请号:US17663293

    申请日:2022-05-13

    Abstract: A method (100) for detecting mycotoxins in organic material, wherein the method (100) comprises of: storing about 2-5 grams of organic material in a temperature less than room temperature; adding a solvent Acetonitrile in the stored organic material to form a first mixture, wherein the first mixture is transferred to a 40-60 ml tubes, centrifuged and shaken for a defined interval; adding 0.5-3 ml of supernatant, 80-120 mg of cyclo-18-carbon (C18) and 200-400 mg of Magnesium sulfate to the first mixture to form a second mixture; and filtering the formed second mixture using a filter syringe to obtain a filtrate, wherein the mycotoxins are detected from the obtained filtrate using Ultra High-Performance Liquid Chromatography (UHPLC) coupled with a mass spectrometer.

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