公开(公告)号：US09347944B2

公开(公告)日：2016-05-24

申请号：US13637815

申请日：2011-03-30

申请人： Mutsunori Shirai ,  Takayuki Ezaki ,  Tsukasa Hayashi ,  Takeshi Ujiiye ,  Makoto Ganaha ,  Shigekazu Yamamoto

发明人： Mutsunori Shirai ,  Takayuki Ezaki ,  Tsukasa Hayashi ,  Takeshi Ujiiye ,  Makoto Ganaha ,  Shigekazu Yamamoto

IPC分类号： C12Q1/68 ,  G01N33/569

CPC分类号： G01N33/56933 ,  C12Q1/6816 ,  C12Q1/6865 ,  C12Q1/689 ,  C12Q2600/112 ,  C12Q2600/156 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56944 ,  Y02A50/451 ,  C12Q2565/137 ,  C12Q2525/143 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/625

摘要： Provided are a method and a kit for accurately and rapidly detecting ten types of targeting pneumonia bacteria: Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, Moraxella catarrhalis, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus. A set of primer pairs directed to their respective target regions contained in the DnaJ gene, etc., of the ten types of pneumonia causative bacteria is designed for the ten bacterial strains and used to amplify gene products. A set of bacterial strain-specific probe pairs is further designed for the ten bacterial strains such that the probe pairs hybridize with the amplification products via sequences in the respective target regions differing from the sequences hybridized by the set of primer pairs. A first probe-bound labeled high molecular carrier in which plural types of first probes for the pneumonia bacteria are bound to a labeled high molecular carrier and a solid-phase second probe-carrying developing support are used as the set of probe pairs to perform nucleic acid chromatography.

摘要翻译： 提供了用于准确和快速检测十种靶向性肺炎细菌的方法和试剂盒：肺炎链球菌，流感嗜血杆菌，肺炎支原体，肺炎衣原体，嗜肺军团菌，肺炎克雷伯菌，铜绿假单胞菌，卡他莫拉菌，耐甲氧西林金黄色葡萄球菌（MRSA） ）和金黄色葡萄球菌（Staphylococcus aureus）。 针对十种类型肺炎致病细菌的DnaJ基因等中包含的各自靶区域的一组引物对设计用于十种细菌菌株并用于扩增基因产物。 针对10个细菌菌株进一步设计了一组细菌菌株特异性探针对，使得探针对通过与由该组引物对杂交的序列不同的各个靶区域中的序列与扩增产物进行杂交。 使用第一探针结合标记的高分子载体，其中用于肺炎细菌的多种类型的第一探针与标记的高分子载体结合，并且使用固相第二探针携带的显影载体作为探针对的一组以进行核酸 酸性色谱。

公开(公告)号：US20130023443A1

公开(公告)日：2013-01-24

申请号：US13637815

申请日：2011-03-30

申请人： Mutsunori Shirai ,  Takayuki Ezaki ,  Tsukasa Hayashi ,  Takeshi Ujiiye ,  Makoto Ganaha ,  Shigekazu Yamamoto

发明人： Mutsunori Shirai ,  Takayuki Ezaki ,  Tsukasa Hayashi ,  Takeshi Ujiiye ,  Makoto Ganaha ,  Shigekazu Yamamoto

IPC分类号： C40B30/04 ,  C40B40/06

CPC分类号： G01N33/56933 ,  C12Q1/6816 ,  C12Q1/6865 ,  C12Q1/689 ,  C12Q2600/112 ,  C12Q2600/156 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56944 ,  Y02A50/451 ,  C12Q2565/137 ,  C12Q2525/143 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/625

摘要： Provided are a method and a kit for accurately and rapidly detecting ten types of targeting pneumonia bacteria: Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, Moraxella catarrhalis, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus. A set of primer pairs directed to their respective target regions contained in the DnaJ gene, etc., of the ten types of pneumonia causative bacteria is designed for the ten bacterial strains and used to amplify gene products. A set of bacterial strain-specific probe pairs is further designed for the ten bacterial strains such that the probe pairs hybridize with the amplification products via sequences in the respective target regions differing from the sequences hybridized by the set of primer pairs. A first probe-bound labeled high molecular carrier in which plural types of first probes for the pneumonia bacteria are bound to a labeled high molecular carrier and a solid-phase second probe-carrying developing support are used as the set of probe pairs to perform nucleic acid chromatography.

摘要翻译： 提供了用于准确和快速检测十种靶向性肺炎细菌的方法和试剂盒：肺炎链球菌，流感嗜血杆菌，肺炎支原体，肺炎衣原体，嗜肺军团菌，肺炎克雷伯菌，铜绿假单胞菌，卡他莫拉菌，耐甲氧西林金黄色葡萄球菌（MRSA） ）和金黄色葡萄球菌（Staphylococcus aureus）。 针对十种类型肺炎致病细菌的DnaJ基因等中包含的各自靶区域的一组引物对设计用于十种细菌菌株并用于扩增基因产物。 针对10个细菌菌株进一步设计了一组细菌菌株特异性探针对，使得探针对通过与由该组引物对杂交的序列不同的各个靶区域中的序列与扩增产物进行杂交。 使用第一探针结合标记的高分子载体，其中用于肺炎细菌的多种类型的第一探针与标记的高分子载体结合，并且使用固相第二探针携带的显影载体作为探针对的一组以进行核酸 酸性色谱。