公开(公告)号：US08609410B2

公开(公告)日：2013-12-17

申请号：US12066313

申请日：2006-09-05

申请人： Mie Nieda ,  Manami Isogai ,  Masashi Takahara ,  Andrew Nicol

发明人： Mie Nieda ,  Manami Isogai ,  Masashi Takahara ,  Andrew Nicol

IPC分类号： C12N5/078 ,  C12N5/0784

CPC分类号： C12N5/0639 ,  A61K38/00 ,  A61K39/0011 ,  A61K2039/5158 ,  C12N5/0636

摘要： Activated antigen-presenting cells that can induce immunocytes including disease antigen-specific CD8+ CTLs and/or γδ T cells efficiently in vivo and/or in vitro, a medical composition comprising the activated antigen-presenting cells, a treatment and prevention method using the activated antigen-presenting cells, and an induction method of immunocytes including disease antigen-specific CTLs and/or γδ T cells induced using the activated antigen-presenting cell, immunocytes induced by the above-noted method, a medical composition comprising the immunocytes, and a treatment and prevention method using the immunocytes are provided. By co-pulsing antigen-presenting cells with bisphosphonate in addition to the pulse with a disease antigen, the ratio of disease antigen-specific CD8+ CTLs and/or γδ T cells and the number of the disease antigen-specific CD8+ CTLs and the γδ T cells can be increased, compared with the case where the co-pulse with bisphosphonate is not carried out.

摘要翻译： 可在体内和/或体外有效诱导包括疾病抗原特异性CD8 + CTL和/或Gammadelta T细胞的免疫细胞的活化抗原呈递细胞，包含活化的抗原呈递细胞的药物组合物，使用活化的抗原呈递细胞的治疗和预防方法 抗原呈递细胞，以及包括使用活化的抗原呈递细胞诱导的疾病抗原特异性CTL和/或Gammadelta T细胞的免疫细胞的诱导方法，由上述方法诱导的免疫细胞，包含免疫细胞的药物组合物和 提供了使用免疫细胞的治疗和预防方法。 除具有疾病抗原的脉冲之外，通过将抗原呈递细胞与双膦酸盐共脉冲，疾病抗原特异性CD8 + CTL和/或Gammadelta T细胞的比例以及疾病抗原特异性CD8 + CTL和gammadelta T的数量 与不进行与双膦酸盐的共脉冲的情况相比，可以增加细胞。

公开(公告)号：US20090104161A1

公开(公告)日：2009-04-23

申请号：US12066313

申请日：2006-09-05

申请人： Mie Nieda ,  Manami Isogai ,  Masashi Takahara ,  Andrew Nicol

发明人： Mie Nieda ,  Manami Isogai ,  Masashi Takahara ,  Andrew Nicol

IPC分类号： A61K45/00 ,  C12N5/08 ,  A61P35/00 ,  C07F9/02

CPC分类号： C12N5/0639 ,  A61K38/00 ,  A61K39/0011 ,  A61K2039/5158 ,  C12N5/0636

摘要： Activated antigen-presenting cells that can induce immunocytes including disease antigen-specific CD8+ CTLs and/or γδ T cells efficiently in vivo and/or in vitro, a medical composition comprising the activated antigen-presenting cells, a treatment and prevention method using the activated antigen-presenting cells, and an induction method of immunocytes including disease antigen-specific CTLs and/or γδ T cells induced using the activated antigen-presenting cell, immunocytes induced by the above-noted method, a medical composition comprising the immunocytes, and a treatment and prevention method using the immunocytes are provided. By co-pulsing antigen-presenting cells with bisphosphonate in addition to the pulse with a disease antigen, the ratio of disease antigen-specific CD8+ CTLs and/or γδ T cells and the number of the disease antigen-specific CD8+ CTLs and the γδ T cells can be increased, compared with the case where the co-pulse with bisphosphonate is not carried out.

摘要翻译： 可在体内和/或体外有效诱导包括疾病抗原特异性CD8 + CTL和/或Gammadelta T细胞的免疫细胞的活化抗原呈递细胞，包含活化的抗原呈递细胞的药物组合物，使用活化的抗原呈递细胞的治疗和预防方法 抗原呈递细胞，以及包括使用活化的抗原呈递细胞诱导的疾病抗原特异性CTL和/或Gammadelta T细胞的免疫细胞的诱导方法，由上述方法诱导的免疫细胞，包含免疫细胞的药物组合物和 提供了使用免疫细胞的治疗和预防方法。 除具有疾病抗原的脉冲之外，通过将抗原呈递细胞与双膦酸盐共脉冲，疾病抗原特异性CD8 + CTL和/或Gammadelta T细胞的比例以及疾病抗原特异性CD8 + CTL和gammadelta T的数量 与不进行与双膦酸盐的共脉冲的情况相比，可以增加细胞。

公开(公告)号：US20070190169A1

公开(公告)日：2007-08-16

申请号：US11631660

申请日：2005-07-07

申请人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

发明人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

IPC分类号： A61K35/32 ,  C12N5/08

CPC分类号： A61K35/15 ,  A61K2035/122 ,  C12N5/0639 ,  C12N2501/999

摘要： The present invention provides dendritic cells capable of efficiently activating and/or proliferating γδ T-cells in vivo and/or in vitro, pharmaceutical compositions comprising said dendritic cells, therapeutic methods and γδ T-cell culture methods utilizing said dendritic cells. By pulsing immature dendritic cells derived from peripheral blood monocytes with a bisphosphonate-based bone metabolism improving drug to enable the cells to activate γδ T-cells, and cultivating them with a cell subset containing γδ T-cells, γδ T-cells can be activated and/or proliferated. This allows for easy proliferation of γδ T-cells without burdening a patient, leading to practical applications of immune cell therapies that utilize γδ T-cells.

摘要翻译： 本发明提供能够在体内和/或体外有效活化和/或增殖Gammadelta T细胞的树突状细胞，包含所述树突状细胞的药物组合物，利用所述树突状细胞的治疗方法和Gammadelta T细胞培养方法。 通过用来自外周血单核细胞的未成熟树突状细胞用基于二膦酸盐的骨代谢改善药物进行脉冲激活，以使细胞能够激活Gammadelta T细胞，并用含有Gammadelta T细胞的细胞亚群进行培养，可以激活Gammadelta T细胞 和/或增殖。 这允许Gammadelta T细胞的容易增殖而不会使患者负担，导致利用Gammadelta T细胞的免疫细胞疗法的实际应用。

公开(公告)号：US08513010B2

公开(公告)日：2013-08-20

申请号：US11631660

申请日：2005-07-07

申请人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

发明人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

IPC分类号： C12N5/0784

CPC分类号： A61K35/15 ,  A61K2035/122 ,  C12N5/0639 ,  C12N2501/999

摘要： The present invention provides dendritic cells capable of efficiently activating and/or proliferating γδ T-cells in vivo and/or in vitro, pharmaceutical compositions comprising said dendritic cells, therapeutic methods and γδ T-cell culture methods utilizing said dendritic cells. By pulsing immature dendritic cells derived from peripheral blood monocytes with a bisphosphonate-based bone metabolism improving drug to enable the cells to activate γδ T-cells, and cultivating them with a cell subset containing γδ T-cells, γδ T-cells can be activated and/or proliferated. This allows for easy proliferation of γδ T-cells without burdening a patient, leading to practical applications of immune cell therapies that utilize γδ T-cells.

摘要翻译： 本发明提供能够在体内和/或体外有效活化和/或增殖Gammadelta T细胞的树突状细胞，包含所述树突状细胞的药物组合物，利用所述树突状细胞的治疗方法和Gammadelta T细胞培养方法。 通过用来自外周血单核细胞的未成熟树突状细胞用基于二膦酸盐的骨代谢改善药物进行脉冲激活，以使细胞能够激活Gammadelta T细胞，并用含有Gammadelta T细胞的细胞亚群进行培养，可以激活Gammadelta T细胞 和/或增殖。 这允许Gammadelta T细胞的容易增殖而不会使患者负担，导致利用Gammadelta T细胞的免疫细胞疗法的实际应用。