公开(公告)号：US20070190169A1

公开(公告)日：2007-08-16

申请号：US11631660

申请日：2005-07-07

申请人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

发明人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

IPC分类号： A61K35/32 ,  C12N5/08

CPC分类号： A61K35/15 ,  A61K2035/122 ,  C12N5/0639 ,  C12N2501/999

摘要： The present invention provides dendritic cells capable of efficiently activating and/or proliferating γδ T-cells in vivo and/or in vitro, pharmaceutical compositions comprising said dendritic cells, therapeutic methods and γδ T-cell culture methods utilizing said dendritic cells. By pulsing immature dendritic cells derived from peripheral blood monocytes with a bisphosphonate-based bone metabolism improving drug to enable the cells to activate γδ T-cells, and cultivating them with a cell subset containing γδ T-cells, γδ T-cells can be activated and/or proliferated. This allows for easy proliferation of γδ T-cells without burdening a patient, leading to practical applications of immune cell therapies that utilize γδ T-cells.

摘要翻译： 本发明提供能够在体内和/或体外有效活化和/或增殖Gammadelta T细胞的树突状细胞，包含所述树突状细胞的药物组合物，利用所述树突状细胞的治疗方法和Gammadelta T细胞培养方法。 通过用来自外周血单核细胞的未成熟树突状细胞用基于二膦酸盐的骨代谢改善药物进行脉冲激活，以使细胞能够激活Gammadelta T细胞，并用含有Gammadelta T细胞的细胞亚群进行培养，可以激活Gammadelta T细胞 和/或增殖。 这允许Gammadelta T细胞的容易增殖而不会使患者负担，导致利用Gammadelta T细胞的免疫细胞疗法的实际应用。

公开(公告)号：US08513010B2

公开(公告)日：2013-08-20

申请号：US11631660

申请日：2005-07-07

申请人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

发明人： Mie Nieda ,  Manami Isogai ,  Kazuhiro Kakimi

IPC分类号： C12N5/0784

CPC分类号： A61K35/15 ,  A61K2035/122 ,  C12N5/0639 ,  C12N2501/999

摘要： The present invention provides dendritic cells capable of efficiently activating and/or proliferating γδ T-cells in vivo and/or in vitro, pharmaceutical compositions comprising said dendritic cells, therapeutic methods and γδ T-cell culture methods utilizing said dendritic cells. By pulsing immature dendritic cells derived from peripheral blood monocytes with a bisphosphonate-based bone metabolism improving drug to enable the cells to activate γδ T-cells, and cultivating them with a cell subset containing γδ T-cells, γδ T-cells can be activated and/or proliferated. This allows for easy proliferation of γδ T-cells without burdening a patient, leading to practical applications of immune cell therapies that utilize γδ T-cells.

摘要翻译： 本发明提供能够在体内和/或体外有效活化和/或增殖Gammadelta T细胞的树突状细胞，包含所述树突状细胞的药物组合物，利用所述树突状细胞的治疗方法和Gammadelta T细胞培养方法。 通过用来自外周血单核细胞的未成熟树突状细胞用基于二膦酸盐的骨代谢改善药物进行脉冲激活，以使细胞能够激活Gammadelta T细胞，并用含有Gammadelta T细胞的细胞亚群进行培养，可以激活Gammadelta T细胞 和/或增殖。 这允许Gammadelta T细胞的容易增殖而不会使患者负担，导致利用Gammadelta T细胞的免疫细胞疗法的实际应用。