公开(公告)号：US20080261264A1

公开(公告)日：2008-10-23

申请号：US11663613

申请日：2005-09-19

申请人： Manuel Araujo Prado ,  Manuel Joaquin Garrido Vazquez ,  Mariano Julio Gomez Lopez

发明人： Manuel Araujo Prado ,  Manuel Joaquin Garrido Vazquez ,  Mariano Julio Gomez Lopez

IPC分类号： C12Q1/04

CPC分类号： C12N1/20

摘要： The invention relates to a culture medium for the detection of Clostridium perfringens. The invention consists in: dissolving protease peptone, microbiological peptone, yeast extract, sodium bisulphite and ferric ammonium citrate in deonized water; sterilising said solution at 121° C. for 15 minutes and cooling same; and, subsequently, adding cycloserine and disodic salt of 4-methylumbelliferyl phosphate, dissolved in deionized water and sterilised by means of filtration. The inventive culture medium is distributed into 150 ml nominal capacity bottles, at a proportion of 50 ml, for refrigerated storage and use within 15 days of the preparation date. The invention is suitable for use in the detection of Clostridium perfringens in water following the inoculation of a 100 ml sample in a bottle containing 50 ml of medium and incubation at 45° C. for between 18 and 20 hours in aerobic conditions.

摘要翻译： 本发明涉及用于检测产气荚膜梭菌的培养基。 本发明包括：将蛋白胨，微生物蛋白胨，酵母提取物，亚硫酸氢钠和柠檬酸铁铵溶解在去离子水中; 在121℃下将所述溶液灭菌15分钟并冷却; 然后加入溶解在去离子水中的4-甲基伞形磷酸酯的环丝氨酸和二盐，并通过过滤灭菌。 将本发明的培养基分配到150ml标称容量的瓶中，比例为50ml，用于在准备日期的15天内进行冷藏和使用。 本发明适用于在含有50ml培养基的瓶中接种100毫升样品后在水中检测产气荚膜梭菌，并在有氧条件下在45℃下孵育18至20小时。