公开(公告)号：US06989252B2

公开(公告)日：2006-01-24

申请号：US09748690

申请日：2000-12-22

申请人： Anastasios Melis ,  Liping Zhang ,  John R. Benemann ,  Marc Forestier ,  Maria Ghirardi ,  Michael Seibert

发明人： Anastasios Melis ,  Liping Zhang ,  John R. Benemann ,  Marc Forestier ,  Maria Ghirardi ,  Michael Seibert

IPC分类号： C12P3/00 ,  C12N1/12

CPC分类号： C12N1/12 ,  C12P3/00 ,  Y10S435/946

摘要： A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.

摘要翻译： 可逆的生理过程提供了微生物中的氧释放和氢产生的时间分离，其包括在照明条件下在培养基中培养微生物培养物以累积内源底物的步骤，从培养基中消耗选自 由硫，铁和/或锰组成的组，将培养物与大气氧密封，将培养物轻轻孵育，由此光诱导的氧产生速率等于或小于呼吸速率，并收集放出的气体。 该方法特别有用于在微生物培养物中实现持续的光生物氢气生产，例如莱茵衣藻（Chlamydomonas reinhardtii）。