公开(公告)号：US20060153854A1

公开(公告)日：2006-07-13

申请号：US11267935

申请日：2005-11-04

申请人： Neelima Bhat ,  Marcia Bieber ,  Nelson Teng ,  Martin Sanders

发明人： Neelima Bhat ,  Marcia Bieber ,  Nelson Teng ,  Martin Sanders

IPC分类号： A61K39/395 ,  C07K16/30

CPC分类号： C07K16/2896 ,  A61K31/475 ,  A61K31/704 ,  A61K38/50 ,  A61K39/3955 ,  A61K39/39558 ,  A61K2039/505 ,  C07K16/28 ,  C07K2317/21

摘要： Compositions and methods for inducing cell membrane wounding, cell permeabilization and cell killing are provided. The composition comprises a polyvalent agent that binds to a highly expressed cell surface antigen present on the surface of a cell. Preferably, the cell surface antigen is associated with the cytoskeleton of the cell. A preferred polyvalent agent is an IgM, and enhanced cell wounding and killing can be provided by the addition of a crosslinking agent. At sublethal concentrations in vivo, the cell wounding antibodies permeabilize cells and dramatically enhance response to chemotherapeutic agents, even in patients refractory to the chemotherapeutic agents.

公开(公告)号：US5437987A

公开(公告)日：1995-08-01

申请号：US077295

申请日：1993-06-14

申请人： Nelson N. H. Teng ,  Marcia Bieber ,  Neelima M. Bhat

发明人： Nelson N. H. Teng ,  Marcia Bieber ,  Neelima M. Bhat

IPC分类号： G01N33/50 ,  G01N33/53

CPC分类号： G01N33/5002 ,  Y10S436/811

摘要： A method for separating nucleated fetal red blood cells and nucleated fetal cells and maternal granular sites from maternal blood is achieved by applying maternal blood to a triple gradient gel, isolating nucleated fetal cells from the gel and binding the isolated fetal cells to a solid support by means of an anti-i antibody bound to the solid support. The separated fetal cells can then be subjected to analysis for fetal sex or genetic disorders.

摘要翻译： 通过将母体血液应用于三重梯度凝胶，从凝胶中分离成核胎儿细胞并将分离的胎儿细胞与固体支持物结合，从而实现从母体血液中分离有核的胎儿红细胞和有核胎儿细胞和母体粒细胞的方法 与固体支持物结合的抗-I抗体的方法。 然后分离的胎儿细胞可以进行胎儿性别或遗传疾病的分析。

公开(公告)号：US20050112130A1

公开(公告)日：2005-05-26

申请号：US10982698

申请日：2004-11-05

申请人： Neelima Bhat ,  Marcia Bieber ,  Nelson Teng ,  Martin Sanders

发明人： Neelima Bhat ,  Marcia Bieber ,  Nelson Teng ,  Martin Sanders

IPC分类号： A61K39/395 ,  A61K39/42 ,  C07K16/28 ,  C12N20060101

CPC分类号： A61K45/06 ,  A61K31/475 ,  A61K39/395 ,  A61K2039/505 ,  A61K2039/507 ,  C07K16/28 ,  C07K16/2887 ,  C07K16/3061 ,  C07K2317/21 ,  A61K2300/00

摘要： Formulations and methods of treating human patients suffering from a condition characterized by lymphoid cancer, autoimmune disease or B cell hyperproliferation are disclosed, the treatment comprising administering (1) a cytotoxic amount of an antibody having specific binding for CDIM epitopes on a B cell, and (2) a cytotoxic agent, including a chemotherapeutic agent, radioactive isotope, cytotoxic antibody, immunoconjugate, ligand conjugate, immunosuppressant, cell growth regulator and/or inhibitor, toxin, or mixtures thereof, including agents that disrupt the cytoskeleton of B cells, particularly vinca alkaloids or colchicine.

摘要翻译： 公开了治疗患有以淋巴癌，自身免疫疾病或B细胞过度增殖为特征的病症的患者的制剂和方法，所述方法包括在B细胞上施用（1）细胞毒性量的具有CDIM表位特异性结合的抗体，以及 （2）细胞毒剂，包括化学治疗剂，放射性同位素，细胞毒性抗体，免疫缀合物，配体结合物，免疫抑制剂，细胞生长调节剂和/或抑制剂，毒素或其混合物，包括破坏B细胞的细胞骨架的试剂，特别是 长春花生物碱或秋水仙碱。