公开(公告)号：US08367387B2

公开(公告)日：2013-02-05

申请号：US12532870

申请日：2008-03-31

申请人： Marek Jan Pecyna ,  Kirk Matthew Schnorr ,  René Ullrich ,  Katrin Scheibner ,  Martin Gunter Kluge ,  Martin Hofrichter

发明人： Marek Jan Pecyna ,  Kirk Matthew Schnorr ,  René Ullrich ,  Katrin Scheibner ,  Martin Gunter Kluge ,  Martin Hofrichter

IPC分类号： C12N9/08 ,  C12N9/02 ,  C12N1/20 ,  C12N1/00 ,  C12P17/12 ,  C12P21/00 ,  C12P19/34 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12N9/0065

摘要： The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.

摘要翻译： 本发明涉及具有过氧化酶活性的多肽和包含这些多肽的组合物，其编码多核苷酸，表达载体和包含此类多核苷酸或载体的重组宿主细胞，产生多肽的方法，以及其应用及其用途的方法，包括 通过在至少一种式（I）的存在下将式（I）的N-杂环与过氧化物酶多肽进行转化，将通式（I）的N-杂环的酶，区域选择性氧合反应至相应的式（II）的N-氧化物 氧化剂在一个阶段的反应过程中。

公开(公告)号：US20100279366A1

公开(公告)日：2010-11-04

申请号：US12532870

申请日：2008-03-31

申请人： Marek Jan Pecyna ,  René Ullrich ,  Kirk Matthew Schnorr ,  Karin Scheibner ,  Martin Gunter Kluge ,  Martin Hofrichter

发明人： Marek Jan Pecyna ,  René Ullrich ,  Kirk Matthew Schnorr ,  Karin Scheibner ,  Martin Gunter Kluge ,  Martin Hofrichter

IPC分类号： C12P17/12 ,  C12N9/08 ,  C12N15/53 ,  C12N1/00

CPC分类号： C12N9/0065

摘要： The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process

摘要翻译： 本发明涉及具有过氧化酶活性的多肽和包含这些多肽的组合物，其编码多核苷酸，表达载体和包含此类多核苷酸或载体的重组宿主细胞，产生多肽的方法，以及其应用及其用途的方法，包括 通过在至少一种式（I）的存在下将式（I）的N-杂环与过氧化物酶多肽进行转化，将通式（I）的N-杂环的酶，区域选择性氧合反应至相应的式（II）的N-氧化物 氧化剂在一个阶段的反应过程中