公开(公告)号：US20140170675A1

公开(公告)日：2014-06-19

申请号：US14124313

申请日：2012-05-31

Applicant: Ron Naaman ,  Eyal Capua ,  Danny Bavli ,  Maria Tkachev

Inventor： Ron Naaman ,  Eyal Capua ,  Danny Bavli ,  Maria Tkachev

IPC: G01N27/414

CPC classification number: G01N27/4145

Abstract: The invention provides a semiconductor device for the detection of an active site-containing protein or a ligand thereof in a solution, said device comprising at least one insulating or semi-insulating layer; at least one conducting semiconductor layer, two conducting pads on top of the upper layer making electrical contact with said at least one conducting semi-conductor layer, such that electrical current can flow between them at a finite distance from the surface of the device; a protective molecular layer fabricated on top of said upper layer and protecting said layer from corrosion; and said ligand or active site-containing protein linked to said protective molecular layer. Exposure of said ligand or active site-containing protein to a solution containing said active site-containing protein or ligand, respectively, causes a current change through the device when a constant electric potential is applied between the two conducting pads. The semi-conductor device can be seen as a molecularly controlled semiconductor resistor (MOCSER) protein sensor based on doped and undoped GaAs stack structure. The GaAs is protected against etching in aqueous environments by the protective molecular layer.

Abstract translation: 本发明提供一种用于在溶液中检测含活性位点的蛋白质或其配体的半导体器件，所述器件包括至少一个绝缘或半绝缘层; 至少一个导电半导体层，在上层的顶部上的两个导电焊盘与所述至少一个导电半导体层电接触，使得电流可以在它们之间在与器件表面有限距离的位置之间流动; 在所述上层顶部制造的保护分子层，并保护所述层免受腐蚀; 和与所述保护性分子层连接的所述配体或含活性位点的蛋白质。 当在两个导电垫之间施加恒定的电位时，所述配体或含活性位点的蛋白质分别暴露于含有所述含活性位点的蛋白质或配体的溶液中，导致通过该装置的电流变化。 半导体器件可以看作是基于掺杂和未掺杂的GaAs堆叠结构的分子控制半导体电阻（MOCSER）蛋白质传感器。 在保护分子层的水溶液环境中保护GaAs不被腐蚀。

公开(公告)号：US08957460B2

公开(公告)日：2015-02-17

申请号：US14124313

申请日：2012-05-31

Applicant: Ron Naaman ,  Eyal Capua ,  Danny Bavli ,  Maria Tkachev

Inventor： Ron Naaman ,  Eyal Capua ,  Danny Bavli ,  Maria Tkachev

IPC: H01L21/00 ,  G01N27/49 ,  H01L27/28 ,  G01N27/414

CPC classification number: G01N27/4145

Abstract: The invention provides a semiconductor device for the detection of an active site-containing protein or a ligand thereof in a solution, said device comprising at least one insulating or semi-insulating layer; at least one conducting semiconductor layer, two conducting pads on top of the upper layer making electrical contact with said at least one conducting semiconductor layer, such that electrical current can flow between them at a finite distance from the surface of the device; a protective molecular layer fabricated on top of said upper layer and protecting said layer from corrosion; and said ligand or active site-containing protein linked to said protective molecular layer. Exposure of said ligand or active site-containing protein to a solution containing said active site-containing protein or ligand, respectively, causes a current change through the device when a constant electric potential is applied between the two conducting pads. The semiconductor device can be seen as a molecularly controlled semiconductor resistor (MOCSER) protein sensor based on doped and undoped GaAs stack structure. The GaAs is protected against etching in aqueous environments by the protective molecular layer.

Abstract translation: 本发明提供一种用于在溶液中检测含活性位点的蛋白质或其配体的半导体器件，所述器件包括至少一个绝缘或半绝缘层; 至少一个导电半导体层，在所述上层顶部上的两个导电焊盘与所述至少一个导电半导体层进行电接触，使得电流可以在它们之间以距离所述器件表面有限的距离流动; 在所述上层顶部制造的保护分子层，并保护所述层免受腐蚀; 和与所述保护性分子层连接的所述配体或含活性位点的蛋白质。 当在两个导电垫之间施加恒定的电位时，所述配体或含活性位点的蛋白质分别暴露于含有所述含活性位点的蛋白质或配体的溶液中，导致通过该装置的电流变化。 半导体器件可以看作是基于掺杂和未掺杂的GaAs堆叠结构的分子控制半导体电阻（MOCSER）蛋白质传感器。 在保护分子层的水溶液环境中保护GaAs不被腐蚀。