公开(公告)号：US20080044815A1

公开(公告)日：2008-02-21

申请号：US10558863

申请日：2004-05-31

申请人： Paul Michael Watt ,  Richard Hopkins ,  Marie Bogoyevitch

发明人： Paul Michael Watt ,  Richard Hopkins ,  Marie Bogoyevitch

IPC分类号： C12Q1/68

CPC分类号： C12N15/1055 ,  C12Q1/6897 ,  C12Q2565/201

摘要： The present invention provides improved reverse hybrid assay methods for identifying amino acid residues within a protein that are required for its interaction or physical association with another protein, wherein disruption of an interaction between a protein of interest and its binding partner protein is assayed for a library of mutations of said protein of interest, and maintenance of an interaction between the protein of interest and another binding partner is assayed simultaneously in a single step, thereby reducing the incidence of uninformative mutations in the protein of interest that are detected.

摘要翻译： 本发明提供了改进的用于鉴定蛋白质内与氨基酸残基相互作用或与另一种蛋白质相互作用所必需的氨基酸残基的改进的反向杂交测定方法，其中分析目标蛋白质与其结合配偶体蛋白质之间相互作用的破坏， 的所述目的蛋白质的突变，以及在一个步骤中同时测定目的蛋白质和另一个结合配偶体之间的相互作用的维持，从而降低检测到的感兴趣的蛋白质中的不知情突变的发生率。