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    Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products
    2.
    发明授权
    Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products 有权
    哺乳动物双杂交系统用于筛选代谢产物积累的调节剂

    公开(公告)号：US07358086B2

    公开(公告)日：2008-04-15

    申请号：US10896600

    申请日：2004-07-21

    Applicant: Osamu Tetsu ,  Kenichi Wakita ,  Frank McCormick

    Inventor： Osamu Tetsu ,  Kenichi Wakita ,  Frank McCormick

    IPC: C12N5/22

    CPC classification number: C12N15/1055 ,  A61K48/00 ,  A61K48/0058 ,  C12Q1/6897 ,  C12Q2565/201

    Abstract: This invention utilizes a two-hybrid system to screen for agents that modulate the ability of a cell to degrade or to accumulate a metabolic product or to selective kill a cell or to selectively express a gene or cDNA in a cell that has a defect in its ability to degrade or to accumulate a metabolic product. One embodiment provides a mammalian cell comprising a nucleic acid encoding a peptide binding domain and an effector gene; a first chimeric protein comprising a nucleic acid binding domain that binds the peptide binding domain attached to the metabolic product or to a ligand that binds to the metabolic product; and a second chimeric protein comprising an expression control protein attached to the metabolic product or to the ligand that binds to the metabolic product such that when the first chimeric protein comprises the metabolic product, the second chimeric protein comprises the ligand and when the first chimeric protein comprises the ligand, the second chimeric protein comprises the metabolic product. The cell is contacted with a test agent and an alteration of expression of the effector gene is detected (if present) where a change in expression of the effector gene in response to the test agent indicates that the test agent modulates the ability of the cell to accumulate or degrade the metabolic product.

    Abstract translation: 本发明利用双杂交系统筛选调节细胞降解能力或积累代谢产物或选择性杀死细胞或选择性地在其中具有缺陷的细胞中表达基因或cDNA的试剂 降解或积累代谢产物的能力。 一个实施方案提供了哺乳动物细胞，其包含编码肽结合结构域和效应基因的核酸; 第一嵌合蛋白，其包含结合与所述代谢产物连接的肽结合结构域的核酸结合结构域或与所述代谢产物结合的配体; 以及第二嵌合蛋白，其包含与代谢产物连接的表达控制蛋白或与所述代谢产物结合的配体，使得当所述第一嵌合蛋白包含所述代谢产物时，所述第二嵌合蛋白包含所述配体，并且当所述第一嵌合蛋白 包含配体，第二嵌合蛋白包含代谢产物。 细胞与测试剂接触，检测效应基因表达的改变（如果存在），其中效应基因响应于测试试剂的表达变化表明测试试剂调节细胞的能力 积累或降解代谢产物。

    Method of identifying compounds that modulate interaction of androgen receptor with beta-catenin
    4.
    发明申请
    Method of identifying compounds that modulate interaction of androgen receptor with beta-catenin 审中-公开
    鉴定调节雄激素受体与β-连环蛋白相互作用的化合物的方法

    公开(公告)号：US20060269950A1

    公开(公告)日：2006-11-30

    申请号：US11435947

    申请日：2006-05-17

    Applicant: Edward Kilbourne ,  Thomas Berrodin

    Inventor： Edward Kilbourne ,  Thomas Berrodin

    IPC: C12Q1/68 ,  C12Q1/66

    CPC classification number: G01N33/5023 ,  C12N15/1086 ,  C12Q1/6897 ,  G01N33/5041 ,  G01N33/743 ,  C12Q2565/201

    Abstract: Methods for determining if test compounds are able to modulate the interaction between androgen receptor and β-catenin are disclosed. Methods for the determining whether a test compound selectively modulates an androgen receptor signaling pathway over a β-catenin-Wnt signaling pathway or a β-catenin-Wnt signaling pathway over an androgen receptor signaling pathway are also disclosed.

    Abstract translation: 公开了确定测试化合物是否能够调节雄激素受体和β-连环蛋白之间相互作用的方法。 还公开了用于确定测试化合物是否选择性调节雄激素受体信号通路上的β-连环蛋白-Wnt信号传导途径或β-连环蛋白-Wnt信号传导途径的雄激素受体信号传导途径的方法。

    In vitro protein interaction detection systems
    6.
    发明授权
    In vitro protein interaction detection systems 有权
    体外蛋白质相互作用检测系统

    公开(公告)号：US06951725B2

    公开(公告)日：2005-10-04

    申请号：US10176826

    申请日：2002-06-20

    Applicant: Markus Kurz ,  Peter Lohse

    Inventor： Markus Kurz ,  Peter Lohse

    IPC: C12Q1/68

    CPC classification number: C12Q1/6897 ,  C12N15/1055 ,  C12N15/1062 ,  C12N15/1075 ,  C12Q2565/201 ,  C12Q2525/203

    Abstract: Disclosed herein are in vitro assays for the identification of interactions between proteins or other molecules, the identification of transcriptional activator proteins, and the detection of compounds that inhibit protein/protein or protein/compound interactions. Also disclosed herein are in vitro assays for the selection of interacting proteins and transcriptional activator proteins out of libraries.

    Abstract translation: 本文公开了用于鉴定蛋白质或其他分子之间的相互作用的体外测定，转录激活蛋白的鉴定以及抑制蛋白质/蛋白质或蛋白质/化合物相互作用的化合物的检测。 本文还公开了用于从文库中选择相互作用蛋白和转录激活蛋白的体外测定。

    Methods and compositions for treating aids and HIV-related disorders using 9145, 1725, 311, 837, 58305, 156, 14175, 50352, 32678, 5560, 7240, 8865, 12396, 12397, 13644, 19938, 2077, 1735, 1786, 10220, 17822, 33945, 43748, 47161, 81982 or 46777
    8.
    发明申请
    Methods and compositions for treating aids and HIV-related disorders using 9145, 1725, 311, 837, 58305, 156, 14175, 50352, 32678, 5560, 7240, 8865, 12396, 12397, 13644, 19938, 2077, 1735, 1786, 10220, 17822, 33945, 43748, 47161, 81982 or 46777 审中-公开
    使用9145,1725,311,837,58305,156,14175,50352,32678,5560,7240,8865,12396,12397,13644,19938,2077,1735,1786,179,195,1735,1786,1735,1735,1746,169,179,169,179,169,179， 10220，17822，33945，43748，47161，81982或46777

    公开(公告)号：US20040180332A1

    公开(公告)日：2004-09-16

    申请号：US10789241

    申请日：2004-02-27

    Applicant: Millennium Pharmaceuticals, Inc.

    Inventor： Douglas M. Powell

    IPC: C12Q001/70

    CPC classification number: C12Q1/6818 ,  C12Q2565/201 ,  C12Q2563/131

    Abstract: The present invention relates to methods for the diagnosis and treatment of AIDS or an HIV-related disorder or disorders. Specifically, the present invention identifies the differential expression of 9145, 1725, 311, 837, 58305, 156, 14175, 50352, 32678, 5560, 7240, 8865, 12396, 12397, 13644, 19938, 2077, 1735, 1786, 10220, 17822, 33945, 43748, 47161, 81982 and 46777 genes in tissues relating to AIDS or an HIV-related disorder, relative to their expression in normal, or non-AIDS or HIV-related disease states, and/or in response to manipulations relevant to AIDS or an HIV-related disorder. The present invention describes methods for the diagnostic evaluation and prognosis of various HIV-related disorders, and for the identification of subjects exhibiting a predisposition to such conditions. The invention also provides methods for identifying a compound capable of modulating AIDS or an HIV-related disorder or disorders. The present invention also provides methods for the identification and therapeutic use of compounds as treatments of AIDS or an HIV-related disorder.

    Abstract translation: 本发明涉及用于诊断和治疗AIDS或HIV相关疾病或病症的方法。 具体地说，本发明鉴定了9145,1725,311,837,58305,156,14175,50352,32678,5560,7240,8865,12396,12397,13644,1993,22077,1735,1786,1020,209的差异表达， 相对于其在正常或非AIDS或HIV相关疾病状态中的表达，和/或响应于相关操作相关的AIDS或HIV相关疾病组织中的17822,3945,43848,47161,81982和46777个基因 艾滋病或艾滋病毒相关疾病。 本发明描述了用于各种HIV相关疾病的诊断评价和预后的方法，以及用于鉴定表现出这种病症倾向的受试者。 本发明还提供了鉴定能够调节艾滋病或HIV相关疾病或病症的化合物的方法。 本发明还提供了用于鉴定和治疗性使用化合物作为AIDS或HIV相关病症治疗的方法。

    Oligonucleotide identifiers
    9.
    发明申请
    Oligonucleotide identifiers 审中-公开
    寡核苷酸识别符

    公开(公告)号：US20020164611A1

    公开(公告)日：2002-11-07

    申请号：US10004275

    申请日：2001-11-15

    Inventor： R. Shoshana Bamdad ,  Cynthia C. Bamdad

    IPC: C12Q001/68 ,  C12M001/34

    CPC classification number: C40B30/04 ,  B82Y30/00 ,  C12N15/1055 ,  C12Q1/6804 ,  C12Q1/6834 ,  G01N33/532 ,  G01N33/58 ,  G01N33/6842 ,  G01N33/6845 ,  C12Q2565/201 ,  C12Q2563/179 ,  C12Q2563/131 ,  C12Q2563/149 ,  C12Q2563/137

    Abstract: Methods, assays, and components are described in which biological samples can be rapidly and sensitively analyzed for the presence of species associated with neurodegenerative disease. Techniques and components are provided for diagnosis of disease, as well as for screening of candidate drugs for treatment of neurodegenerative disease. The techniques are simple, extremely sensitive, and utilize readily-available components. Binding species, capable of binding a neurodegenerative disease aggregate-forming or aggregate-forming species, are fastened to surfaces of electrodes and surfaces of particles, or provided free in solution, to bind aggregate-forming species and/or be involved in aggregation.

    Abstract translation: 描述了方法，测定和组分，其中可以快速和敏感地分析生物样品中与神经变性疾病相关的物种的存在。 提供技术和组件用于诊断疾病，以及筛选用于治疗神经变性疾病的候选药物。 这些技术简单，极其敏感，并且利用易于使用的组件。 能够结合神经变性疾病聚集体形成或聚集形成物质的结合物质被紧固到电极和颗粒表面的表面，或者在溶液中自由形成，从而结合聚集体形成物质和/或参与聚集。

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