公开(公告)号：US20070240527A1

公开(公告)日：2007-10-18

申请号：US11285839

申请日：2005-11-23

申请人： Calum MacAulay ,  Jagoda Korbelik ,  Mark Cardeno

发明人： Calum MacAulay ,  Jagoda Korbelik ,  Mark Cardeno

IPC分类号： G01N1/00 ,  G01N1/02

CPC分类号： B01L3/0265 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00367 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00531 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01L3/021 ,  B01L3/0262 ,  B01L3/0275 ,  B01L2400/0633 ,  B82Y30/00 ,  C40B60/14 ,  G01N33/80 ,  Y10T436/2575

摘要： Microvolume liquid dispensers that provide simple and inexpensive approaches to making cytology microarrays. In some embodiments, the dispensers comprise tips that comprise an outer sleeve, typically shaped like a funnel, that holds a reciprocating needle or pin. The tip of the pin slightly extends beyond the distal opening of the outer sleeve in one position, and is retracted in another position. When the pin is in the distal position the pin contacts the inner surfaces of the sleeve and blocks cytology liquid from flowing through the opening of the sleeve. Thus the pin and sleeve cooperate to form a reservoir behind the blockage. When the pin is pushed up into the sleeve, for example by touching the tip to a glass slide, a passage is formed between the outer surface of the pin and the inner surface of the sleeve. The liquid in the reservoir then flows through the passage and onto the slide. Removing the tip from the substrate moves the pin back to its original position, re-forming the reservoir and leaving a predetermined microvolume amount of the liquid on the slide.

摘要翻译： 微量液体分配器提供简单和便宜的方法来制备细胞学微阵列。 在一些实施例中，分配器包括尖端，其包括保持往复式针或销的通常形状为漏斗的外套筒。 销的尖端在一个位置稍微延伸超过外套筒的远端开口，并且缩回到另一位置。 当销处于远端位置时，销接触套筒的内表面并阻止细胞液体流过套筒的开口。 因此，销和套筒协作以在堵塞物后面形成储存器。 当销被推入套筒时，例如通过将尖端触及玻璃滑块，在销的外表面和套筒的内表面之间形成通道。 然后，储存器中的液体流过通道并流到载玻片上。 从基板移除尖端将销移回到其初始位置，重新形成储存器并在载玻片上留下预定的微量的液体。