公开(公告)号：US6022951A

公开(公告)日：2000-02-08

申请号：US628540

申请日：1996-04-10

申请人： Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

发明人： Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

IPC分类号： A61K38/00 ,  C07K14/36 ,  C12N1/21 ,  C12N15/31 ,  G01N33/538

CPC分类号： G01N33/538 ,  C07K14/36 ,  A61K38/00 ,  G01N2333/465 ,  Y10S530/808 ,  Y10S530/81

摘要： The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.

摘要翻译： 本发明涉及链霉抗生物素蛋白和具有改变的物理性质例如增加的稳定性或增加或降低的结合生物素的亲和力的肽。 本发明还涉及使用链霉亲和素蛋白或肽检测，鉴定，分离和分离靶的方法。 具有增加或降低亲和力的链霉亲和素允许使用链霉抗生物素蛋白 - 生物素偶联系统进行检测和分离系统，其中必须除去一种或另一种结合配偶体。 这样的系统可用于纯化功能性蛋白质和活细胞。 本发明还涉及编码这些链霉抗生物素蛋白和肽以及含有这些核酸的重组细胞如细菌，酵母和哺乳动物细胞的核酸。