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1.发明授权Assay for nucleotide triphosphate using a modified nucleoside diphosphate kinase comprising a fluorescent label 有权使用包含荧光标记的修饰的核苷二磷酸激酶测定核苷酸三磷酸公开(公告)号：US07297506B2
公开(公告)日：2007-11-20
申请号：US10779718
申请日：2004-02-18
摘要： Nucleoside diphosphates (NDPs) are detected or measured by following the dephosphorylation of the phosphoenzyme form of nucleoside diphosphate kinase (NDPK), and nucleoside triphosphates (NTPs) are detected or measured by following the phosphorylation of NDPK to its phosphoenozyme form. A typical process involves (a) causing NDP in sample to bind to NDPK phosphoenzyme, or causing NTP in sample to phosphorylate NDPK and (b) detecting a change in a characteristic of the enzyme which differs between its phosphorylated and unphosphorylated forms. This may be aided by labelling the enzyme. Quantitative data can be obtained. Both in vivo and in vitro measurements can be made.
摘要翻译： 通过跟踪磷酸酶形式的核苷二磷酸激酶（NDPK）的去磷酸化来检测或测量核苷二磷酸（NDP），并通过遵循NDPK磷酸化为磷酸酶形式来检测或测量核苷三磷酸（NTPs）。 典型的方法包括：（a）使样品中的NDP与NDPK磷酸酶结合，或使样品中的NTP磷酸化NDPK，和（b）检测其磷酸化和非磷酸化形式之间不同的酶的特征变化。 这可以通过标记酶来辅助。 可以获得定量数据。 可以进行体内和体外测量。
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2.发明授权Assay for nucleoside diphosphate using a modified nucleoside diphosphate kinase comprising a fluorescent label 有权使用包含荧光标记的修饰的核苷二磷酸激酶测定核苷二磷酸公开(公告)号：US06746849B1
公开(公告)日：2004-06-08
申请号：US09937296
申请日：2001-11-14
IPC分类号： C12Q148
摘要： Nucleoside diphosphates (NDPs) are detected or measured by following the dephosphorylation of the phosphoenzyme form of nucleoside diphosphate kinase (NDPK), and nucleoside triphosphates (NTPs) are detected or measured by following the phosphorylation of NDPK to its phosphoenzyme form. A typical process involves (a) causing NDP in sample to bind to NDPK phosphoenzyme, or causing NTP in sample to phosphorylate NDPK and (b) detecting a change in a characteristic of the enzyme which differs between its phosphorylated and unphosphorylated forms. This may be aided by labelling the enzyme. Quantitative data can be obtained. Both in vivo and in vitro measurements can be made.
摘要翻译： 通过跟踪磷酸酶形式的核苷二磷酸激酶（NDPK）的去磷酸化来检测或测量核苷二磷酸（NDP），并且通过遵循NDPK磷酸化至其磷酸酶形式来检测或测量核苷三磷酸（NTPs）。 典型的方法包括：（a）使样品中的NDP与NDPK磷酸酶结合，或使样品中的NTP磷酸化NDPK，和（b）检测其磷酸化和非磷酸化形式之间不同的酶的特征变化。 这可以通过标记酶来辅助。 可以获得定量数据。 可以进行体内和体外测量。
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