High sensitivity assay method
    2.
    发明授权
    High sensitivity assay method 失效
    高灵敏度测定方法

    公开(公告)号:US4556634A

    公开(公告)日:1985-12-03

    申请号:US506844

    申请日:1983-06-22

    IPC分类号: C12Q1/00 C12Q1/26 C12Q1/32

    CPC分类号: C12Q1/008

    摘要: A high sensitivity assay method of the coenzyme cyclic assay type, eliminates the need for a separate indication reaction, by using the cycling reaction itself as the indication reaction, according to the following steps: ##STR1## the thus-formed formazan is then colorimetrically measured, to give a highly sensitive determination of the quantity of the substance to be assayed, e.g. NAD.sup.+, NADP.sup.+, malic acid or .gamma.-aminobutyric acid in human serum.

    摘要翻译: 根据以下步骤,通过使用循环反应本身作为指示反应,辅酶循环测定型的高灵敏度测定方法不需要单独的指示反应:(1)主反应体系:其中AH2 或A:要测定的物质; NAD(P)+:NAD +或NADP +; NAD(P)H:还原形式的NAD +或NADP +; (2)分解反应:中和:碱中和:其中M:碱金属和R:低级烷基; (3)循环反应:然后比色测定如此形成的甲,,以测定待测物质的量的高灵敏度。 NAD +,NADP +,苹果酸或γ-氨基丁酸。