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公开(公告)号：US09001321B2
公开(公告)日：2015-04-07
申请号：US13578164
申请日：2011-02-08
CPC分类号： G02B21/002 , G01N2021/653 , G01N2021/655 , G02B2207/114
摘要： Provided is a microscope and an observation method which can improve spatial resolution. A microscope according to an aspect of the invention includes a laser light source (10), an objective lens (16) that focuses light from the laser light source on a sample, and a detector (22) that detects the laser light as signal light from a sample (17) when the sample (17) is irradiated with the laser light. The light is applied to the sample with an intensity changed to obtain a nonlinear region where intensities of the light and the signal light have a nonlinear relation due to occurrence of saturation or nonlinear increase of the signal light when the light has a maximum intensity, and the detector (22) detects the signal light according to the intensity of the laser light to perform observation based on a saturation component or a nonlinear increase component of the signal light.
摘要翻译： 提供了可以提高空间分辨率的显微镜和观察方法。 根据本发明的一个方面的显微镜包括激光源（10），将来自激光光源的光聚焦在样本上的物镜（16）以及检测器（22），其将激光检测为信号光 来自样品（17）当样品（17）被激光照射时。 将光以强度变化的方式施加到样品，以获得非线性区域，其中当光具有最大强度时，光和信号光的强度由于饱和度的发生或信号光的非线性增加而具有非线性关系，以及 检测器（22）根据激光的强度检测信号光，以基于信号光的饱和分量或非线性增加分量进行观察。
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公开(公告)号：US20120307238A1
公开(公告)日：2012-12-06
申请号：US13578164
申请日：2011-02-08
CPC分类号： G02B21/002 , G01N2021/653 , G01N2021/655 , G02B2207/114
摘要： Provided is a microscope and an observation method which can improve spatial resolution. A microscope according to an aspect of the invention includes a laser light source (10), an objective lens (16) that focuses light from the laser light source on a sample, and a detector (22) that detects the laser light as signal light from a sample (17) when the sample (17) is irradiated with the laser light. The light is applied to the sample with an intensity changed to obtain a nonlinear region where intensities of the light and the signal light have a nonlinear relation due to occurrence of saturation or nonlinear increase of the signal light when the light has a maximum intensity, and the detector (22) detects the signal light according to the intensity of the laser light to perform observation based on a saturation component or a nonlinear increase component of the signal light.
摘要翻译： 提供了可以提高空间分辨率的显微镜和观察方法。 根据本发明的一个方面的显微镜包括激光源（10），将来自激光光源的光聚焦在样本上的物镜（16）以及检测器（22），其将激光检测为信号光 来自样品（17）当样品（17）被激光照射时。 将光以强度变化的方式施加到样品，以获得非线性区域，其中当光具有最大强度时，光和信号光的强度由于饱和度的发生或信号光的非线性增加而具有非线性关系，以及 检测器（22）根据激光的强度检测信号光，以基于信号光的饱和分量或非线性增加分量进行观察。
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