公开(公告)号：US06171798B2

公开(公告)日：2001-01-09

申请号：US09442039

申请日：1999-11-17

申请人： Arnold L. Levine ,  Maureen Elizabeth Murphy ,  David H. Mack ,  Kurt Carlyle Gish ,  Edward Yat Wah Tom

发明人： Arnold L. Levine ,  Maureen Elizabeth Murphy ,  David H. Mack ,  Kurt Carlyle Gish ,  Edward Yat Wah Tom

IPC分类号： C12Q168

CPC分类号： G01N33/57484 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/136

摘要： Many genes are identified as being p53-regulated which were not heretofore known to be p53-regulated. This includes both genes whose expression is induced and genes whose expression is repressed by the expression of wild-type p53. Monitoring expression of these genes is used to provide indications of p53 status in a cell. Such monitoring can also be used to screen for useful anti-cancer therapeutics, as well as for substances which are carcinogenic. Defects in p53 can be bypassed by supplying p53 induced genes to cells. Defects in p53 can also be bypassed by supplying antisense constructs to p53-repressed genes.

摘要翻译： 许多基因被鉴定为p53调节的，这些基因迄今尚未被p53调节。 这包括其表达被诱导的基因和通过野生型p53的表达抑制其表达的基因。 监测这些基因的表达用于提供细胞中p53状态的指示。 此类监测也可用于筛选有用的抗癌治疗剂，以及用于致癌物质。 可以通过向细胞提供p53诱导的基因来绕过p53中的缺陷。 通过向p53抑制的基因提供反义构建体也可以绕过p53中的缺陷。

公开(公告)号：US6020135A

公开(公告)日：2000-02-01

申请号：US49025

申请日：1998-03-27

申请人： Arnold J. Levine ,  Maureen Elizabeth Murphy ,  David H. Mack ,  Kurt Carlyle Gish ,  Edward Yat Wah Tom

发明人： Arnold J. Levine ,  Maureen Elizabeth Murphy ,  David H. Mack ,  Kurt Carlyle Gish ,  Edward Yat Wah Tom

IPC分类号： G01N33/50 ,  A61K31/7105 ,  A61K48/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/574 ,  C17Q1/68 ,  C07H21/04

CPC分类号： G01N33/57484 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/136

摘要： Many genes are identified as being p53-regulated which were not heretofore known to be p53-regulated. This includes both genes whose expression is induced and genes whose expression is repressed by the expression of wild-type p53. Monitoring expression of these genes is used to provide indications of p53 status in a cell. Such monitoring can also be used to screen for useful anti-cancer therapeutics, as well as for substances which are carcinogenic. Defects in p53 can be bypassed by supplying p53 induced genes to cells. Defects in p53 can also be bypassed by supplying antisense constructs to p53-repressed genes.

摘要翻译： 许多基因被鉴定为p53调节的，这些基因迄今尚未被p53调节。 这包括其表达被诱导的基因和通过野生型p53的表达抑制其表达的基因。 监测这些基因的表达用于提供细胞中p53状态的指示。 此类监测也可用于筛选有用的抗癌治疗剂，以及用于致癌物质。 可以通过向细胞提供p53诱导的基因来绕过p53中的缺陷。 通过向p53抑制的基因提供反义构建体也可以绕过p53中的缺陷。