公开(公告)号：US06841535B2

公开(公告)日：2005-01-11

申请号：US09915914

申请日：2001-07-26

申请人： Gilles Divita ,  May C. Morris ,  Jean Mery ,  Frederic Heitz ,  Joseph Fernandez ,  John Archdeacon ,  Kyle Horndorp

发明人： Gilles Divita ,  May C. Morris ,  Jean Mery ,  Frederic Heitz ,  Joseph Fernandez ,  John Archdeacon ,  Kyle Horndorp

IPC分类号： A61K47/48 ,  C07K7/08 ,  C07K14/00 ,  C12N15/87 ,  A61K38/10

CPC分类号： C07K7/08 ,  A61K47/64 ,  C07K14/001 ,  C12N15/87

摘要： Peptides that are useful as transfection agents are described. The peptides can facilitate the efficient cellular internalization of a broad range and size of compounds that when non-covalently complexed therewith are efficiently internalized into a cell. Advantages include but are not limited to excellent transfection efficiency, relatively low toxicity, internalization by a broad host cell spectrum, and the simplicity and cost-effectiveness that arise from not having to covalently complex the peptide with a specific molecule to be delivered. Applications include but are not limited to the delivery of diagnostics and therapeutics, as well as drug discovery, gene discovery, and the analysis and/or manipulation of other cellular and molecular biological functions. Claims are made for transfection agents, compositions of matter, including pharmaceutical compositions, reagent kits, methods of delivery, and methods of identification of additional peptides for performing and/or including in the same.

摘要翻译： 描述了可用作转染剂的肽。 这些肽可以促进广泛范围和大小的化合物的有效细胞内化，当与其非共价复合时可有效地内化到细胞中。 优点包括但不限于优异的转染效率，相对低的毒性，广泛的宿主细胞谱的内化，以及不必将肽与待递送的特定分子共价复合而产生的简单性和成本效益。 应用包括但不限于诊断和治疗的传递，以及药物发现，基因发现以及其他细胞和分子生物功能的分析和/或操作。 对于转染剂，物质组合物，包括药物组合物，试剂盒，递送方法以及用于鉴定用于进行和/或包含在其中的另外的肽的方法而提出要求。