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    Bacterial inosine 5′-monophosphate dehydrogenase (“IMPDH”) DNA as a dominant selectable marker in mammals and other eukaryotes
    1.
    发明授权
    Bacterial inosine 5′-monophosphate dehydrogenase (“IMPDH”) DNA as a dominant selectable marker in mammals and other eukaryotes 有权
    细菌肌苷5'-单磷酸脱氢酶（“IMPDH”）DNA作为哺乳动物和其他真核生物中的主要选择标记

    公开(公告)号：US07183106B1

    公开(公告)日：2007-02-27

    申请号：US10856409

    申请日：2004-05-28

    申请人： Eliezer Huberman ,  Mekhine J. Baccam

    发明人： Eliezer Huberman ,  Mekhine J. Baccam

    IPC分类号： C12N15/00

    CPC分类号： C12N9/0006

    摘要： The present invention relates to a nucleic acid sequence and its corresponding protein sequence useful as a dominant selectable marker in eukaryotes. More specifically the invention relates to a nucleic acid encoding a bacterial IMPDH gene that has been engineered into a eukaryotic expression vectors, thereby permitting bacterial IMPDH expression in mammalian cells. Bacterial IMPDH expression confers resistance to MPA which can be used as dominant selectable marker in eukaryotes including mammals. The invention also relates to expression vectors and cells that express the bacterial IMPDH gene as well as gene therapies and protein synthesis.

    摘要翻译： 本发明涉及可用作真核生物中显性选择标记的核酸序列及其相应的蛋白质序列。 更具体地，本发明涉及编码细菌IMPDH基因的核酸，其已经被工程化成真核表达载体，从而允许在哺乳动物细胞中表达细菌IMPDH。 细菌IMPDH表达赋予对MPA的抗性，其可以用作包括哺乳动物在内的真核生物的显性选择标记。 本发明还涉及表达细菌IMPDH基因以及基因治疗和蛋白质合成的表达载体和细胞。