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    Conotoxin peptides
    1.
    发明授权
    Conotoxin peptides 失效
    芋螺毒素肽

    公开(公告)号：US5719264A

    公开(公告)日：1998-02-17

    申请号：US319554

    申请日：1994-10-07

    申请人： Ki-Joon Shon ,  Baldomero M. Olivera ,  J. Michael McIntosh ,  Arik Hasson ,  Micha E. Spira

    发明人： Ki-Joon Shon ,  Baldomero M. Olivera ,  J. Michael McIntosh ,  Arik Hasson ,  Micha E. Spira

    IPC分类号： C07K14/435 ,  A61K38/00 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00

    CPC分类号： C07K14/43504

    摘要： The present invention is directed to conotoxin peptides having 25-35 amino acids, six cysteines which form three disulfide bonds between the first and fourth, second and fifth, and third and sixth cysteines, respectively. The invention is directed to .delta.-conotoxin GmVIA having the formula Val-Lys-Pro-Cys-Arg-Lys-Glu-Gly-Gln-Leu-Cys-Asp-Pro-Ile-Phe-Gln-Asn-Cys-Cys-Arg-Gly-Trp-Asn-Cys-Val-Leu-Phe-Cys-Val (SEQ ID NO:1). This peptide activates sodium channels. The invention is further directed to .mu.O-conotoxin peptides of the generic formula Ala-Cys-Xaa.sub.1 -Lys-Lys-Trp-Glu-Tyr-Cys-Ile-Val-Pro-Ile-Xaa.sub.2 -Gly-Phe-Xaa.sub.3 -Tyr-Cys-Cys-Pro-Gly-Leu-Ile-Cys-Gly-Pro-Phe-Val-Cys-Val, wherein Xaa.sub.1 is Arg or Ser, Xaa.sub.2 is Ile or Leu and Xaa.sub.3 is Ile or Val (SEQ ID NO:2). These peptides block sodium charmels. Examples of .mu.O-conotoxin peptides of the present invention are MrVIA, having the formula Ala-Cys-Arg-Lys-Lys-Trp-Glu-Tyr-Cys-Ile-Val-Pro-Ile-Ile-Gly-Phe-Ile-Tyr-Cys-Cys-Pro-Gly-Leu-Ile-Cys-Gly-Pro-Phe-Val-Cys-Val (SEQ ID NO:3), and MrVIB, having the formula Ala-Cys-Ser-Lys-Lys-Trp-Glu-Tyr-Cys-Ile-Val-Pro-Ile-Leu-Gly-Phe-Val-Tyr-Cys-Cys-Pro-Gly-Leu-Ile-Cys-Gly-Pro-Phe-Val-Cys-Val (SEQ ID NO:4).

    摘要翻译： 本发明涉及分别在第一和第四，第二和第五以及第三和第六半胱氨酸之间形成25-35个氨基酸的唾液毒素肽，六个半胱氨酸形成三个二硫键。 本发明涉及具有式Val-Lys-Pro-Cys-Arg-Lys-Glu-Gly-Gln-Leu-Cys-Asp-Pro-Ile-Phe-Gln-Asn-Cys-Cys- Arg-Gly-Trp-Asn-Cys-Val-Leu-Phe-Cys-Val（SEQ ID NO：1）。 该肽激活钠通道。 本发明进一步涉及通式为Ala-Cys-Xaa1-Lys-Lys-Trp-Glu-Tyr-Cys-Ile-Val-Pro-Ile-Xaa2-Gly-Phe-Xaa3-Tyr的mu O-芋螺毒素肽 -Cys-Cys-Pro-Gly-Leu-Ile-Cys-Gly-Pro-Phe-Val-Cys-Val，其中Xaa1是Arg或Ser，Xaa2是Ile或Leu，Xaa3是Ile或Val（SEQ ID NO： 2）。 这些肽阻断钠的魅力。 本发明的mu O-芋螺毒素肽的实例​​是具有式Ala-Cys-Arg-Lys-Lys-Trp-Glu-Tyr-Cys-Ile-Val-Pro-Ile-Gly-Phe-Ile的MrVIA -Tyr-Cys-Cys-Pro-Gly-Leu-Ile-Cys-Gly-Pro-Phe-Val-Cys-Val（SEQ ID NO：3）和具有式Ala-Cys-Ser-Lys- Lys-Trp-Glu-Tyr-Cys-Ile-Val-Pro-Ile-Leu-Gly-Phe-Val-Tyr-Cys-Cys-Pro-Gly-Leu-Ile-Cys-Gly-Pro-Phe-Val- Cys-Val（SEQ ID NO：4）。