公开(公告)号：US4307191A

公开(公告)日：1981-12-22

申请号：US130482

申请日：1980-03-31

申请人： Miodrag Ristic ,  Michael G. Levy

发明人： Miodrag Ristic ,  Michael G. Levy

IPC分类号： A61K39/018 ,  C12N1/10 ,  C12Q1/18

CPC分类号： A61K39/018 ,  C12N1/10 ,  C12Q1/18 ,  Y10S435/947

摘要： A method for quantitative propagation of hemotropic parasites, especially Babesia parasites, in vitro, Babesia-infected erythrocyte cultures are incubated in an environment of enhanced carbon dioxide tension wherein the culture medium overlay provides a barrier to oxygen transmission such that cellular hemoglobin is essentially maintained in its deoxy state. By varying environmental carbon dioxide tension, merozoite stages of the parasite are selectively reversibly induced to migration between residence in host cells or culture medium, rendering possible the isolation of infective antigenic merozoites in large quantities. Specific Babesia antigen is harvested in large quantity from the in vitro culture medium.

摘要翻译： 血液寄生虫，特别是巴贝虫寄生虫，体外，巴贝虫感染的红细胞培养物的定量增殖的方法在增强的二氧化碳张力的环境中温育，其中培养基覆盖物提供对氧传递的屏障，使得细胞血红蛋白基本上保持在 其脱氧状态。 通过改变环境二氧化碳的张力，寄生虫的裂殖子阶段被选择性地可逆地诱导在宿主细胞或培养基中的迁移之间，从而可能大量分离感染性抗原性裂殖子。 从体外培养基中大量收获特异性巴贝虫抗原。