公开(公告)号：US12129509B2

公开(公告)日：2024-10-29

申请号：US17241991

申请日：2021-04-27

申请人： Avails Medical, Inc.

发明人： Oren S. Knopfmacher ,  Meike Herget ,  Nitin K. Rajan ,  Ashraf M. Wahba

IPC分类号： C12Q1/18 ,  G01N27/30 ,  G01N27/327

CPC分类号： C12Q1/18 ,  G01N27/3275 ,  G01N27/302

摘要： Various methods, devices, and systems for determining the susceptibility of infectious agents to anti-infectives are disclosed herein. A method comprises introducing an inoculum solution comprising the infectious agent into a sample receiving space of a diagnostic device. The sample receiving space comprises a plurality of growth control wells devoid of the anti-infective and a plurality of active electrode wells comprising the anti-infective at differing concentrations. A water immiscible liquid can be introduced into the sample receiving space to seal the plurality of wells and the diagnostic device can be incubated for a period of time. The minimum inhibitory concentration (MIC) of the anti-infective on the infectious agent can be determined by monitoring and comparing one or more solution characteristics of the inoculum solution within the active electrode wells with the one or more solution characteristics of the inoculum solution with the growth control wells.

公开(公告)号：US20240344106A1

公开(公告)日：2024-10-17

申请号：US18751532

申请日：2024-06-24

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

发明人： Shaopeng WANG ,  Fenni ZHANG

IPC分类号： C12Q1/06 ,  C12Q1/18 ,  G06T7/00 ,  G06T7/246

CPC分类号： C12Q1/06 ,  C12Q1/18 ,  G06T7/0016 ,  G06T7/246 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30241

摘要： Provided herein are methods of assessing the presence of microbes in a liquid sample that include assessing an initial integrated scattering intensity of objects (IC0) in the sample and an integrated scattering intensity of the objects at a time t (ICt) from modified images of the liquid sample, and identifying the sample as comprising microbes for (ICt)/(IC0) above a predefined infection threshold TI. Related systems and other aspects are also provided.

公开(公告)号：US20240344104A1

公开(公告)日：2024-10-17

申请号：US18389382

申请日：2023-11-14

申请人： Spectral Platforms, Inc.

发明人： Ravi Verma

IPC分类号： C12Q1/04 ,  C12Q1/18 ,  G01N21/31

CPC分类号： C12Q1/04 ,  C12Q1/18 ,  G01N21/31

摘要： Provided are methods of separating an absorption spectrum into a Rayleigh scattering contribution and an absorption contribution. By performing such separations and removing the influence of Rayleigh scattering, the absorption of a sample can be more accurately measured. Provided are additional methods that involve separating an absorption spectrum into a Rayleigh scattering contribution and an absorption contribution: assessing whether or not a microorganism is present in a biological fluid, assessing the effect of a pharmaceutical drug on a microorganism, and treating a subject suspected of having an infection. Provided are systems and non-transitory computer readable storage media for separating an absorption spectrum into a Rayleigh scattering and an absorption contribution.

公开(公告)号：US20240339218A1

公开(公告)日：2024-10-10

申请号：US18681425

申请日：2022-08-03

申请人： Deepull Diagnostics S.L.

发明人： Jordi CARRERA FABRA ,  Rafael BRU GIBERT ,  Richard Max IVEY

IPC分类号： G16H50/20 ,  C12Q1/18 ,  C12Q1/686 ,  C12Q1/689 ,  G01N21/65 ,  G16B25/20 ,  G16B40/10 ,  G16H10/60 ,  G16H20/10

CPC分类号： G16H50/20 ,  C12Q1/18 ,  C12Q1/686 ,  C12Q1/689 ,  G01N21/65 ,  G16B25/20 ,  G16B40/10 ,  G16H10/60 ,  G16H20/10

摘要： A sepsis detection system includes a first subsystem configured to detect a presence of an infection in a patient, a second subsystem configured to detect a presence of a dysregulated host response in the patient, a third subsystem configured to detect organ dysfunction in the patient, a fourth subsystem configured to detect antimicrobial resistance (AMR) of a pathogen in the patient, and a processing device. The first, second, third, and fourth subsystems and the processing device are communicatively coupled together via a network. The processing device is configured to determine a presence of sepsis in the patient based on the presence of the infection, the presence of the dysregulated host response, and clinical data indicative of the organ dysfunction in the patient. The subsystems utilize at least one of polymerase chain reaction (PCR) processing. Raman spectroscopy, clinical data, electronic health record (EHR) data, and antimicrobial susceptibility testing (AST).

公开(公告)号：US20240318225A1

公开(公告)日：2024-09-26

申请号：US18293305

申请日：2022-07-29

申请人： General Electric Company ,  The Penn State Research Foundation

发明人： Christopher Michael Puleo ,  Erik Leeming Kvam ,  Pak Kin Wong ,  Peter Torab ,  Christine Lynne Surrette

IPC分类号： C12Q1/24 ,  C12Q1/18 ,  G01N33/49

CPC分类号： C12Q1/24 ,  C12Q1/18 ,  G01N33/491

摘要： The subject matter of the present disclosure generally relates to techniques for isolating bacterial cells from a biological sample comprising red blood cells. Using an aggregating agent and an anticoagulant during sedimentation permits separation of bacterial pathogens in the sample from red blood cells. The separated sedimentation layer, which is enriched in any bacterial pathogens, can be centrifuged and resuspended to concentrate the bacteria for additional analysis, such as bacterial identification and/or antibiotic susceptibility tests.

公开(公告)号：US20240309422A1

公开(公告)日：2024-09-19

申请号：US18604656

申请日：2024-03-14

申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  UNIVERSITE GRENOBLE ALPES ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT POLYTECHNIQUE DE GRENOBLE

发明人： Pierre MARCOUX ,  Yoann ROUPIOZ ,  Marc ZELSMANN ,  Emmanuel PICARD ,  Larry O’CONNELL

IPC分类号： C12Q1/18 ,  C12M1/00 ,  C12M1/32 ,  C12Q1/70

CPC分类号： C12Q1/18 ,  C12M23/12 ,  C12M23/22 ,  C12M23/34 ,  C12M41/06 ,  C12Q1/70

摘要： A method for detecting a lytic activity of one or more types of phages or of the activity of one or more antibiotics with respect to a bacterial strain, the method including using a first multiwell plate, each well receiving a sample containing phages or antibiotics, making at least one deposit on a transparent area of a support, the deposit containing bacteria of the bacterial strain, applying the first plate against the support so that each sample in each well comes into contact with the deposit, for each well, determining, using the detection means, the activity or absence of activity of the phage or antibiotic with respect to the bacterial strain, taking into account the intensity of light signals transmitted through each well and/or the variation thereof over time.

公开(公告)号：US20240294992A1

公开(公告)日：2024-09-05

申请号：US18358437

申请日：2023-07-25

申请人： The United States of America, as represented by the Secretary of Agriculture ,  UNIVERSITY OF VIRGINIA PATENT FOUNDATION

发明人： JOSEPH A CAPOBIANCO, JR. ,  JOSEPH LEE ,  CHIN YI CHEN ,  YIPING HE ,  CHERYL M ARMSTRONG ,  SUE A REED ,  BRYAN BERGER

IPC分类号： C12Q1/689 ,  C12Q1/18 ,  C12Q1/28 ,  C12Q1/6851

CPC分类号： C12Q1/689 ,  C12Q1/18 ,  C12Q1/28 ,  C12Q1/6851

摘要： Provided herein are compositions and methodologies for identifying microbes in biofilms and adhered to biotic and abiotic surfaces, utilizing enzymes that degrade biofilms and disperse aggregated clusters of microorganisms. Utilizing enzymes such as CAase to degrade biofilms, organisms released from biofilms are identified more readily than from untreated biofilms. Biofilms from a variety of sources, both biotic and abiotic, can be analyzed utilizing the present disclosure.

公开(公告)号：US12061143B2

公开(公告)日：2024-08-13

申请号：US16477260

申请日：2018-01-12

申请人： Georgia Tech Research Corporation

发明人： Robert Martin Dickson ,  Tzu-Hsueh Huang

IPC分类号： C12Q1/18 ,  G01N15/14 ,  G01N21/39 ,  G01N15/10

CPC分类号： G01N15/1459 ,  C12Q1/18 ,  G01N21/39 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1488

摘要： The invention is directed to a method for rapidly determining antibiotic susceptibility in bodily fluid samples based on rigorous multidimensional statistical metrics. In some embodiments, the method incorporates flow cytometry to determine susceptibility. In preferred embodiments, the method is adapted for use with samples with low bacterial counts.

公开(公告)号：US20240263213A1

公开(公告)日：2024-08-08

申请号：US18683730

申请日：2022-08-16

申请人： NAGI BIOSCIENCE SA

发明人： Matteo CORNAGLIA ,  Fabien TACHE

IPC分类号： C12Q1/18 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36

CPC分类号： C12Q1/18 ,  C12M23/34 ,  C12M29/06 ,  C12M41/46 ,  C12M41/48

摘要： Microscopic worm culture and observation apparatus (1) comprising a support structure (10), one or more chip holders (3) mounted on the support structure, a pump (P) and a valve system (V), each chip holder configured for holding a microfluidic chip (2) having one or more microfluidic channels (54) and culture chambers (52) therein extending between a pump side coupling (44a) of the microfluidic chip and a reservoir side coupling (44b) of the microfluidic chip. The support structure comprises a reservoirs support platform (7) mounted on a movable table (12), the reservoirs support platform (7) configured for holding a plurality of nutrition reservoirs (5) for containing microscopic worms or nutrients and substances to be tested in a liquid. The chip holder (3) and/or microfluidic chips (2) comprise reservoir side fluidic couplings (26) in the form of hollow tubes extending from the microfluidic chip (2) to a tip (26b) at a free end of the hollow tube, each tip (26b) insertable in a corresponding nutrition reservoir (5). The support structure comprises an enclosure (15) forming a chamber within which the one or more chip holders and the reservoirs support platform is housed, and a temperature control unit (19) and a temperature sensor (23) for control of the temperature within the chamber, the enclosure comprising an openable or removable cover (17) allowing access to the inside of the enclosure.

公开(公告)号：US20240240226A1

公开(公告)日：2024-07-18

申请号：US18550786

申请日：2022-04-08

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Jianghong RAO ,  Jinghang XIE ,  Ran MU

IPC分类号： C12Q1/18 ,  C07D265/38 ,  C07D477/14 ,  C07D501/36 ,  C12Q1/34 ,  G01N21/64

CPC分类号： C12Q1/18 ,  C07D265/38 ,  C07D477/14 ,  C07D501/36 ,  C12Q1/34 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2333/986

摘要： A dual-caged fluorogenic resorufin probe (CDA) has been developed that is stable under physiological condition with low background but becomes highly fluorescent upon β-lactamase/esterase activation and further oxidation. The probes of the disclosure are advantageous for initial screening of broad-spectrum β-lactam antibiotics resistance and carbapenem resistant pathogens at diagnosis. After a two-step filtration, the assay of the disclosure can report 103 c.f.u./mL cephalosporin- and carbapenem-resistant bacteria in urine within 2 hours at room temperature.