Method and algorithm for quantifying polynucleotides
    2.
    发明授权
    Method and algorithm for quantifying polynucleotides 有权
    定量多核苷酸的方法和算法

    公开(公告)号:US07739054B2

    公开(公告)日:2010-06-15

    申请号:US11474698

    申请日:2006-06-22

    IPC分类号: G01N33/50 C12Q1/68

    摘要: Machine executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.

    摘要翻译: 分析生长曲线数据的机器可执行方法,以确定从基线阶段到增长阶段的过渡。 该方法的应用包括分析核酸扩增反应中扩增子合成时间依赖性监测的结果,以量化测试样品中核酸模板的起始量。 该方法有利地通过避免建立用于计算生长阶段的起始,计算导数或执行线性回归分析的阈值的需要来简化定量。