公开(公告)号：US07026527B2

公开(公告)日：2006-04-11

申请号：US09989339

申请日：2002-01-28

申请人： Saverio Carl Falco ,  Omolayo O. Famodu ,  Jan Antoni Rafalski ,  Michael Lee Ramaker ,  Mitchell Christian Tarczynski ,  Catherine Thorpe

发明人： Saverio Carl Falco ,  Omolayo O. Famodu ,  Jan Antoni Rafalski ,  Michael Lee Ramaker ,  Mitchell Christian Tarczynski ,  Catherine Thorpe

IPC分类号： A01H1/00 ,  A01H4/00 ,  C12N15/82 ,  C12N5/10 ,  C07H21/04

CPC分类号： C12N9/1007 ,  C12N15/8253

摘要： This invention relates to a nucleic acid fragment encoding a plant 5-methyltetra-hydropteroyltriglutamate-homocysteine methyltransferase or methionine synthase. The invention also includes chimeric genes, a first encoding a plant methionine synthase (MS) gene, a second encoding a plant cystathionine γ-synthase (CS) gene, a third encoding feedback-insensitive aspartokinase (AK) or bifunctional feedback-insensitive aspartokinase-homoserine dehydrogenase (AK-HDH), which is operably linked to a plant chloroplast transit sequence, and a fourth encoding a methionine-rich protein, all operably linked to plant seed-specific regulatory sequences. Methods for their use to produce increased levels of methionine in the seeds of transformed plants are provided.