公开(公告)号：US20050221477A1

公开(公告)日：2005-10-06

申请号：US10507343

申请日：2002-03-11

申请人： Hideo Ema ,  Hiromitsu Nakauchi ,  Mitsujiro Osawa

发明人： Hideo Ema ,  Hiromitsu Nakauchi ,  Mitsujiro Osawa

IPC分类号： A61P43/00 ,  C07K14/47 ,  C12N5/0789 ,  C12N5/08 ,  C07K7/06

CPC分类号： C12N5/0647 ,  C07K14/47 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2501/415

摘要： The sustaining stem cells for example, hematopoietic stem cells while holding in the undifferentiated state and maintaining pluripotency by providing a polypeptide having a WIF domain for example, WIF-1 to the stem cells has been successfully achieved. This polypeptide preferably also contains an EGF-like repeat. This result has been achieved by providing a polypeptide preferably having the WIF domain together with a stem cell survival factor such as SCF. Thus, it becomes possible to easily provide a large amount of stem cells for example, hematopoietic stem cells containing functional precursor cells. It has also become possible to more efficiently transfer a gene into the stem cells. Because it is homogeneous and almost free from impurities, the stem cell composition can remarkably relieve side effects for example, infection, which occur in conventional therapies using hematopoietic stem cells.

摘要翻译： 维持性干细胞例如造血干细胞同时保持未分化状态并通过向干细胞提供具有WIF结构域例如WIF-1的多肽来保持多能性已被成功地实现。 该多肽优选还含有EGF样重复。 该结果已经通过提供优选具有WIF结构域的多肽与干细​​胞存活因子如SCF来实现。 因此，可以容易地提供大量的干细胞，例如含有功能性前体细胞的造血干细胞。 也可以更有效地将基因转移到干细胞中。 因为它是均质的并且几乎没有杂质，干细胞组合物可以显着地减轻使用造血干细胞的常规疗法中发生的副作用，例如感染。